Tripartite Chromatin Localization of Budding Yeast Shugoshin Involves Higher-Ordered Architecture of Mitotic Chromosomes

G3 : Genes - Genomes - Genetics
Xiexiong Deng, Min-Hao Kuo

Abstract

The spindle assembly checkpoint (SAC) is key to faithful segregation of chromosomes. One requirement that satisfies SAC is appropriate tension between sister chromatids at the metaphase-anaphase juncture. Proper tension generated by poleward pulling of mitotic spindles signals biorientation of the underlying chromosome. In the budding yeast, the tension status is monitored by the conserved Shugoshin protein, Sgo1p, and the tension sensing motif (TSM) of histone H3. ChIP-seq reveals a unique TSM-dependent, tripartite domain of Sgo1p in each mitotic chromosome. This domain consists of one centromeric and two flanking peaks 3 - 4 kb away, present exclusively in mitosis. Strikingly, this trident motif coincides with cohesin localization, but only at the centromere and the two immediate adjacent loci, despite that cohesin is enriched at numerous regions throughout mitotic chromosomes. Chromosome conformation capture assays reveal apparent looping at the centromeric and pericentric regions. The TSM-Sgo1p-cohesin triad is therefore at the center stage of higher-ordered chromatin architecture for error-free segregation.

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Citations

Oct 2, 2019·Critical Reviews in Biochemistry and Molecular Biology·Josh Lawrimore, Kerry Bloom

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Datasets Mentioned

BETA
GSE110953

Methods Mentioned

BETA
ChIP
PCR
ChIP-seq
immunoprecipitation
for
electrophoresis
two-hybrid

Software Mentioned

Intergrative Genomics Viewer
Bowtie2
HOMER
NIH Image J
Cen

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