Tripartite motif 32 prevents pathological cardiac hypertrophy

Clinical Science
Lijuan ChenHongliang Li

Abstract

TRIM32 (tripartite motif 32) is widely accepted to be an E3 ligase that interacts with and eventually ubiquitylates multiple substrates. TRIM32 mutants have been associated with LGMD-2H (limb girdle muscular dystrophy 2H). However, whether TRIM32 is involved in cardiac hypertrophy induced by biomechanical stresses and neurohumoral mediators remains unclear. We generated mice and isolated NRCMs (neonatal rat cardiomyocytes) that overexpressed or were deficient in TRIM32 to investigate the effect of TRIM32 on AB (aortic banding) or AngII (angiotensin II)-mediated cardiac hypertrophy. Echocardiography and both pathological and molecular analyses were used to determine the extent of cardiac hypertrophy and subsequent fibrosis. Our results showed that overexpression of TRIM32 in the heart significantly alleviated the hypertrophic response induced by pressure overload, whereas TRIM32 deficiency dramatically aggravated pathological cardiac remodelling. Similar results were also found in cultured NRCMs incubated with AngII. Mechanistically, the present study suggests that TRIM32 exerts cardioprotective action by interruption of Akt- but not MAPK (mitogen-dependent protein kinase)-dependent signalling pathways. Additionally, inactivatio...Continue Reading

References

Jul 25, 2000·Circulation·B H Lorell, B A Carabello
Feb 1, 2002·American Journal of Human Genetics·Patrick FroskKlaus Wrogemann
Mar 24, 2005·Annals of Neurology·Benedikt G H SchoserKlaus Wrogemann
Aug 3, 2005·The Journal of Clinical Investigation·Ichiro ShiojimaKenneth Walsh
Oct 18, 2005·Molecular and Cellular Biology·Ji LuoLewis C Cantley
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Annie P ChiangVal C Sheffield
Apr 26, 2006·Circulation·Brian DeBoschAnthony J Muslin
Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Joerg Heineke, Jeffery D Molkentin
Oct 13, 2006·European Journal of Clinical Investigation·T YokotaK Heese
Jan 22, 2009·Human Molecular Genetics·Elena KudryashovaMelissa J Spencer
Apr 8, 2009·Human Molecular Genetics·Matthew LockeDerek J Blake
Dec 9, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Agrawal RohiniRandhir Singh
Jan 8, 2010·The Journal of Investigative Dermatology·Yuangang LiuMolly F Kulesz-Martin
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hongliang LiCongxin Huang
Jul 5, 2011·European Journal of Heart Failure·Antoine H Chaanine, Roger J Hajjar
Oct 11, 2011·Journal of Cell Science·Tomonobu SatoShigetsugu Hatakeyama
Dec 21, 2011·Biochemical and Biophysical Research Communications·Tomonobu SatoShigetsugu Hatakeyama
Aug 22, 2012·The Journal of Cell Biology·Shenhav CohenAlfred L Goldberg
Jan 3, 2013·The Journal of Clinical Investigation·Jop H van BerloJeffery D Molkentin
Jan 12, 2013·Basic Research in Cardiology·Jing LuHongliang Li
Feb 26, 2014·The Journal of Cell Biology·Shenhav CohenAlfred L Goldberg
Mar 18, 2015·Circulation·Ryan A Frieler, Richard M Mortensen
Jun 17, 2015·Hypertension·Xiao-Jing ZhangHongliang Li

❮ Previous
Next ❯

Citations

Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alexandra VolodinShenhav Cohen
Feb 10, 2017·The Journal of Physiology·Jennifer E Gilda, Aldrin V Gomes
Apr 6, 2019·Journal of Cellular and Molecular Medicine·Kaowen YanPeifeng Li
Apr 29, 2020·Journal of Cellular and Molecular Medicine·Hui YangHong-Xia Wang
Mar 2, 2019·Journal of Cellular Physiology·Xu ChenQijun Zheng
Aug 17, 2020·Journal of Cellular and Molecular Medicine·Rujia MiaoJiangang Wang
Jan 21, 2021·Biomolecules·Dina Aweida, Shenhav Cohen
Sep 14, 2020·Journal of Molecular and Cellular Cardiology·Thomas G Martin, Jonathan A Kirk

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
PCR
in vitro transcription
electrophoresis
dissection
genotyping

Software Mentioned

Image
Pro Plus
SPSS

Related Concepts

Related Feeds

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.