Tripeptide-copper complex GHK-Cu (II) transiently improved healing outcome in a rat model of ACL reconstruction

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Sai-Chuen FuKai-Ming Chan

Abstract

After anterior cruciate ligament reconstruction (ACLR), the biological healing of the graft is a rate-limiting step which can contribute to graft failure. The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu(II) (GHK-Cu) is a well-known activator of tissue remodeling. We investigated whether GHK-Cu can improve graft healing following ACLR. Seventy-two rats underwent unilateral ACLR were randomized to saline, 0.3 or 3 mg/ml GHK-Cu groups (n = 24). Post-operational intra-articular injections were given from week 2, once a week, for 4 weeks. Gait analysis was performed pre-injury and at harvesting time. At 6 or 12 weeks post-operation, knee specimens were harvested for knee laxity test, graft pull-out test, and histology. At 6 weeks post-ACLR, GHK-Cu groups resulted in a smaller side-to-side difference in knee laxity as compared to the saline group (p = 0.009), but there was no significant difference at 12 weeks post-operation. The graft complex in the 0.3 mg/ml GHK-Cu group had higher stiffness than saline group at 6 weeks post-operation (p = 0.026), but there was no significant difference in ultimate load, gait parameters, and histological scores among treatment groups. All grafts failed mid-substance during pull-out test...Continue Reading

References

Nov 10, 1970·Biochemistry·R C SiegelG R Martin
Jan 1, 1970·Journal of Biomechanics·J B Morrison
Jul 29, 1999·The American Journal of Sports Medicine·S A RodeoR F Warren
Aug 18, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Fu Sai ChuenChan Kai Ming
Nov 5, 2004·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Jit-Kheng LimEng-Hin Lee
Oct 13, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Martha M MurraySusan A Connolly
Oct 9, 2007·Clinics in Sports Medicine·Geoffrey S Baer, Christopher D Harner
Jun 3, 2008·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·S U SchefflerA Weiler
Jul 18, 2008·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Max EkdahlFreddie H Fu
Jul 23, 2008·Journal of Biomaterials Science. Polymer Edition·Loren Pickart
Aug 21, 2008·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Helmut SeitzThomas Nau
Mar 31, 2012·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Sai-Chuen FuKai-Ming Chan
Aug 15, 2012·Osteoarthritis and Cartilage·S C FuK M Chan
Jun 5, 2013·Best Practice & Research. Clinical Rheumatology·Philip Courtney, Michael Doherty
Mar 1, 2014·Orthopaedic Journal of Sports Medicine·Sai-Chuen FuKai-Ming Chan

❮ Previous
Next ❯

Citations

Jun 30, 2016·ACS Chemical Neuroscience·K RajasekharT Govindaraju
May 23, 2017·Tissue Engineering. Part a·Corina Adriana GhebesDaniel B F Saris
Jun 8, 2021·Asia-Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology·Shiyi YaoPatrick Shu-Hang Yung

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved