Triphasic and quadriphasic waveforms are superior to biphasic waveforms for synchronized beating of cardiomyocytes

Journal of Electrocardiology
Lars EnochsonCamilla Karlsson

Abstract

Within pacemaker research few attempts have been made to find an optimal waveform phase sequence that synchronizes beating of cardiomyocytes at an electrode. Multielectrode arrays (MEAs) offer electrophysiological screening of cardiomyocytes serving as a system for preliminary screening of pacing waveform design. The HL-1 cell line was cultured in MEAs until confluence and stimulated with biphasic, triphasic, and quadriphasic waveforms. The amplitudes required for synchronized beating of the cells were determined. Triphasic and quadriphasic waveforms were more efficient in eliciting synchronized beating of the HL-1 cells compared with the biphasic waveform because it allows significant reductions in synchronizing voltage amplitudes and reductions in supplied stimulus. The MEA system allows for a straightforward manner to investigate effects of waveform design on synchronized beating in cardiomyocytes in vitro. Increased number of phase changes in a pacing waveform seems to be the major reason for the reduction in synchronizing amplitudes.

References

May 1, 1976·IEEE Transactions on Bio-medical Engineering·R D Klafter, L Hrebien
Nov 1, 1991·Pacing and Clinical Electrophysiology : PACE·S SaksenaE Burkhardt
Sep 1, 1971·Medical & Biological Engineering·O Z Roy, R W Wehnert
Oct 30, 1969·Annals of the New York Academy of Sciences·V ParsonnetR Avery
Apr 1, 1994·American Heart Journal·S M Blanchard, R E Ideker
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·W C ClaycombN J Izzo
Mar 22, 2000·Circulation·J HuangR E Ideker
Aug 10, 2000·Journal of Interventional Cardiac Electrophysiology : an International Journal of Arrhythmias and Pacing·P F BakkerJ J Bredée
May 1, 2001·IEEE Transactions on Bio-medical Engineering·A V SahakianS Swiryn
May 9, 2001·Pacing and Clinical Electrophysiology : PACE·C C de CockC A Visser
Jun 29, 2001·Experimental Physiology·N ShibataI Kodama
Jan 12, 2002·Journal of Electrocardiology·Wayne C McDanielJack J Curtis
Aug 9, 2003·Journal of the American College of Cardiology·Yi ZhangRichard E Kerber
Aug 19, 2003·Analytical and Bioanalytical Chemistry·Alfred StettHugo Haemmerle
Dec 8, 2004·Cellular and Molecular Life Sciences : CMLS·V Krauthamer, T C Smith
Jun 2, 2007·Journal of Interventional Cardiac Electrophysiology : an International Journal of Arrhythmias and Pacing·Mark W Kroll, Charles D Swerdlow
Oct 27, 2009·Journal of Electrocardiology·S Suave Lobodzinski, Michael Laks

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.