Triphenyl phosphate is a selective PPARγ modulator that does not induce brite adipogenesis in vitro and in vivo.

Archives of Toxicology
Stephanie KimJennifer J Schlezinger

Abstract

Triphenyl phosphate (TPhP) is an environmental PPARγ ligand, and growing evidence suggests that it is a metabolic disruptor. We have shown previously that the structurally similar ligand, tributyltin, does not induce brite adipocyte gene expression. Here, using in vivo and in vitro models, we tested the hypothesis that TPhP is a selective PPARγ ligand, which fails to induce brite adipogenesis. C57BL/6 J male mice were fed either a low or very high-fat diet for 13 weeks. From weeks 7-13, mice were injected intraperitoneally, daily, with vehicle, rosiglitazone (Rosi), or TPhP (10 mg/kg). Compared to Rosi, TPhP did not induce expression of browning-related genes (e.g. Elovl3, Cidea, Acaa2, CoxIV) in mature adipocytes isolated from inguinal adipose. To determine if this resulted from an effect directly on the adipocytes, 3T3-L1 cells and primary human preadipocytes were differentiated into adipocytes in the presence of Rosi or TPhP. Rosi, but not TPhP, induced expression of brite adipocyte genes, mitochondrial biogenesis and cellular respiration. Further, Rosi and TPhP-induced distinct proteomes and phosphoproteomes; Rosi enriched more regulatory pathways related to fatty acid oxidation and mitochondrial proteins. We assessed the r...Continue Reading

References

Apr 1, 1996·The Journal of Nutrition·N MoustaïdJ W Taylor
Jun 18, 2002·Nature Medicine·Norikazu MaedaYuji Matsuzawa
Oct 11, 2003·Developmental Cell·Shamina M RangwalaMitchell A Lazar
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Nov 4, 2005·Histochemistry and Cell Biology·Annette B GallerMatthias Reinhard
Nov 24, 2005·American Journal of Physiology. Endocrinology and Metabolism·Ann V HertzelDavid A Bernlohr
Nov 25, 2005·Diabetes·Bartholomew A PedersonPeter J Roach
Oct 24, 2008·Cell Biochemistry and Biophysics·Kai-Chun Wu, J-P Jin
Dec 2, 2008·Nature·Vikas ChandraFraydoon Rastinejad
Jul 25, 2009·The New England Journal of Medicine·James A Timmons, Bente K Pedersen
Jul 24, 2010·Nature·Jang Hyun ChoiBruce M Spiegelman
Jun 17, 2011·Journal of Cellular Biochemistry·Feng YeJian-Bin Tong
Aug 11, 2011·American Journal of Physiology. Endocrinology and Metabolism·Tomas B WaldénJan Nedergaard
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 10, 2012·Journal of Biochemical and Molecular Toxicology·Heather B PatisaulHeather M Stapleton
May 9, 2013·Nature Medicine·Maryam AhmadianRonald M Evans
Feb 28, 2014·Adipocyte·Matthias Rosenwald, Christian Wolfrum
May 6, 2014·Trends in Endocrinology and Metabolism : TEM·Martina I LefterovaSusanne Mandrup
Jul 26, 2014·Environmental Health Perspectives·Hari K PillaiJennifer J Schlezinger
Oct 16, 2014·Genes & Development·Jang Hyun ChoiBruce M Spiegelman
Nov 20, 2014·Nature·Alexander S BanksBruce M Spiegelman
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Mar 4, 2015·Journal of Medicinal Chemistry·Santiago Garcia-VallvéMiquel Mulero
Aug 20, 2015·The New England Journal of Medicine·Melina ClaussnitzerManolis Kellis
Nov 29, 2015·International Review of Cell and Molecular Biology·Zeyad D Nassar, Marie-Odile Parat
Oct 6, 2016·Pflügers Archiv : European journal of physiology·Yong ChenAlexander Pfeifer
Oct 21, 2016·Reproductive Toxicology·Jerrold J HeindelFrederick Vom Saal
Nov 4, 2016·Nature Protocols·Stefka TyanovaJuergen Cox
Feb 7, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·German Cano-SanchoMichele A La Merrill
Sep 14, 2017·Endocrine Reviews·Michael W SchwartzRudolph L Leibel

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Citations

Jul 30, 2021·Environmental Health Perspectives·Stephanie KimJennifer J Schlezinger

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Datasets Mentioned

BETA
PXD012337

Methods Mentioned

BETA
sumoylation
acetylation
Fluorescence
Assay
ubiquitination

Key Resources (RRID) Mentioned

CVCL_0123

Software Mentioned

MaxQuant
R
LIMMA R
Gene Set Enrichment Analysis ( GSEA )
AutoAnnotate
GSEA
Enrichment Map
JANUS
ImageJ
Prism

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