Triphenyltin Chloride Delays Leydig Cell Maturation During Puberty in Rats

Frontiers in Pharmacology
Linchao LiRen-Shan Ge

Abstract

Triphenyltin chloride (TPT) is present in a wide range of human foods. TPT could disrupt testis function as a potential endocrine disruptor of Leydig cells. However, the effect of TPT on pubertal Leydig cell development is still unclear. The objective of the current study was to explore whether exposure to TPT affected Leydig cell developmental process and to clarify the underlying mechanisms. Male Sprague-Dawley rats at 35 days of age were randomly divided into four groups and received normal corn oil (control), 0.5, 1, or 2 mg/kg/day TPT for 18 days. Immature Leydig cells isolated from 35-day-old rat testes were treated with TPT (10 and 100 nM) for 24 h in vitro. In vivo exposure to ≥0.5 mg/kg TPT lowered serum testosterone levels and lowered Star mRNA. TPT at 2 mg/kg also lowered Lhcgr, Cyp11a1, Hsd3b1, Hsd17b3 as well as pAKT1/AKT1, pAKT2/AKT2, and pERK1/2/ERK1/2 ratios. In vitro exposure to TPT (100 nM) increased ROS production and induced cell apoptosis rate in rat immature Leydig cells. In conclusion, TPT exposure disrupts Leydig cell development possibly via interfering with the phosphorylation of AKT1, AKT2, and ERK1/2 kinases.

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Citations

Aug 28, 2019·International Journal of Toxicology·Derong MaGuoqiang Xue
Feb 19, 2021·Stem Cell Reviews and Reports·Zhuo-Jie LiuZhi-Jun Zang
Jun 7, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xingwang LiRen-Shan Ge

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Methods Mentioned

BETA
PCR
immunoprecipitation assay
Assay
electrophoresis
FACS

Software Mentioned

GraphPad
Image J
GraphPad Prism
Pro Plus
Image

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

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