Triple colour fluorescent in-situ hybridization for chromosomes X,Y and 1 on spare human embryos

Human Reproduction
H LavergeM Dhont

Abstract

The potential for implantation of human embryos obtained by in-vitro fertilization is presumably determined to a large extent by their chromosomal constitution but cytogenetic analysis of preimplantation embryos has been hampered by a number of practical and technical problems. With the advent of fluorescent in-situ hybridization (FISH) a practical method for numerical chromosomal analysis has become available. A limited amount of data has been obtained with FISH on human embryos using probes binding to chromosomes X, Y, 16, 18 and 13/21 combined or for chromosomes X and Y or 1 and 17. It was our purpose to extend these data by the combined analysis of chromosomes X, Y and 1 in spare human embryos. A short fluorescent in-situ hybridization procedure involving the simultaneous use of three deoxyribonucleic acid probes detected with red, green, and a mixture of red and green was used to determine chromosomal abnormalities in 116 spare embryos with a poor morphological score and/or displaying one or more multinucleated blastomeres. The majority of the embryos was obtained by intracytoplasmic sperm injection. Less than half of the embryos (n = 54) were diploid and only 39 of them were uniformly XY11 or XX11; two embryos showed a no...Continue Reading

Citations

Feb 8, 2000·Prenatal Diagnosis·J C Harper, D Wells
Nov 20, 1998·Molecular Reproduction and Development·J KobayashiE Sato
Oct 1, 2005·Journal of Assisted Reproduction and Genetics·Jingly Fung WeierCatherine Racowsky
Dec 21, 2006·Journal of Assisted Reproduction and Genetics·Mónica GilFrancisco Rísquez
Mar 3, 2007·Journal of Assisted Reproduction and Genetics·Elpida Fragouli
Jan 1, 1999·Maturitas·U Eichenlaub-Ritter
Jun 29, 2001·Trends in Molecular Medicine·D Wells, J D Delhanty
Aug 25, 2009·Human Reproduction Update·Victor Y FujimotoRichard W Browne
Oct 6, 2005·Reproductive Biology and Endocrinology : RB&E·Mohamed F M MitwallyMichael P Diamond
Oct 3, 2008·Fertility and Sterility·Shiri Barbash-HazanDalit Ben-Yosef
Jan 8, 2008·Molecular and Cellular Endocrinology·Tsvia FrumkinDalit Ben-Yosef
Jun 16, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Dagan Wells
Dec 15, 2010·Fertility and Sterility·Victor Y FujimotoMina Alikani
May 14, 2016·Journal of Assisted Reproduction and Genetics·Johnny S YounisMoshe Ben-Ami
Jan 18, 2006·Fertility and Sterility·Lora K Shahine, Marcelle I Cedars
Jan 28, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Shalom Bar-AmiMoshe Zilberstein

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