Triple oscillating field technique for accurate distance measurements by solid-state NMR

The Journal of Chemical Physics
Navin Khaneja, Niels C Nielsen

Abstract

We present a new concept for homonuclear dipolar recoupling in magic-angle-spinning (MAS) solid-state NMR experiments which avoids the problem of dipolar truncation. This is accomplished through the introduction of a new NMR pulse sequence design principle: the triple oscillating field technique. We demonstrate this technique as an efficient means to accomplish broadband dipolar recoupling of homonuclear spins, while decoupling heteronuclear dipolar couplings and anisotropic chemicals shifts and retaining influence from isotropic chemical shifts. In this manner, it is possible to synthesize Ising interaction (2IzSz) Hamiltonians in homonuclear spin networks and thereby avoid dipolar truncation--a serious problem essentially all previous homonuclear dipolar recoupling experiments suffer from. Combination of this recoupling concept with rotor assisted dipolar refocusing enables easy readout of internuclear distances through comparison with analytical Fresnel curves. This forms the basis for a new class of solid-state NMR experiments with potential for structure analysis of uniformly 13C labeled proteins through accurate measurement of 13C-13C internuclear distances. The concept is demonstrated experimentally by measurement of C a...Continue Reading

References

Mar 1, 1997·Journal of Magnetic Resonance·M Bak, N C Nielsen
Jul 17, 1998·Nature Structural Biology·R G Griffin
Nov 1, 1985·Physical Review. B, Condensed Matter·D Suter, R R Ernst
Jul 2, 1999·Journal of Magnetic Resonance·P Hodgkinson, L Emsley
Jan 5, 2000·Journal of Magnetic Resonance·B M FungK Ermolaev
Dec 1, 2000·Journal of Magnetic Resonance·M BakN C Nielsen
May 2, 2001·Journal of Magnetic Resonance·R VerelB H Meier
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chad M RienstraRobert G Griffin
Feb 27, 2003·Journal of the American Chemical Society·Philip T F WilliamsonBeat H Meier
Dec 11, 2003·Journal of the American Chemical Society·Ramesh RamachandranRobert G Griffin
Jan 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christopher P JaroniecRobert G Griffin
Jan 22, 2004·Journal of the American Chemical Society·Vladimir Ladizhansky, Robert G Griffin
Mar 16, 2004·Journal of Biomolecular NMR·Evan CrockerSteven O Smith
Jul 23, 2004·The Journal of Chemical Physics·Jerry C C Chan, Robert Tycko
Jun 16, 2005·Journal of the American Chemical Society·Stephan G ZechAnn E McDermott
Aug 12, 2005·Chembiochem : a European Journal of Chemical Biology·Karsten SeidelMarc Baldus
May 30, 2006·The Journal of Chemical Physics·Anant K Paravastu, Robert Tycko
Jan 25, 2007·Journal of the American Chemical Society·Józef R LewandowskiRobert G Griffin
Oct 4, 2007·Physical Chemistry Chemical Physics : PCCP·M CarravettaM H Levitt

❮ Previous
Next ❯

Citations

Sep 18, 2012·The Journal of Chemical Physics·Wei Qiang, Robert Tycko
Jun 18, 2009·The Journal of Chemical Physics·Lasse Arnt StraasøNiels Chr Nielsen
Mar 26, 2009·The Journal of Chemical Physics·Marvin J BayroRobert G Griffin
Jan 7, 2009·The Journal of Chemical Physics·Gaël De PaëpeRobert G Griffin
Mar 13, 2012·Annual Review of Physical Chemistry·Gaël De Paëpe
Dec 17, 2014·The Journal of Chemical Physics·Kazuyuki TakedaK Takegoshi
Jul 21, 2009·Journal of Magnetic Resonance·B HuJ P Amoureux
Jan 25, 2011·Physical Chemistry Chemical Physics : PCCP·Johanna Becker-BaldusSteven P Brown
Sep 3, 2020·The Journal of Chemical Physics·Lokeswara Rao PotnuruVipin Agarwal
Jan 29, 2021·Solid State Nuclear Magnetic Resonance·Yi JiGuangjin Hou
May 16, 2008·Analytical Chemistry·Cecil Dybowski, Shi Bai
Aug 7, 2010·The Journal of Physical Chemistry Letters·Anders B NielsenNiels Chr Nielsen
May 29, 2021·Journal of Magnetic Resonance·Lokeswara Rao PotnuruVipin Agarwal
Sep 25, 2018·The Journal of Physical Chemistry Letters·Nghia Tuan DuongVipin Agarwal

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
irradiating
x-ray diffraction

Software Mentioned

SIMPSON
RADAR
REDOR
TEDOR
REPULSION
SIMMOL
GATE
TOFU

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.