Triplet-triplet energy transfer in Peridinin-Chlorophyll a-protein reconstituted with Chl a and Chl d as revealed by optically detected magnetic resonance and pulse EPR: comparison with the native PCP complex from Amphidinium carterae

Biochimica Et Biophysica Acta
Marilena Di ValentinDonatella Carbonera

Abstract

The triplet state of the carotenoid peridinin, populated by triplet-triplet energy transfer from photoexcited chlorophyll triplet state, in the reconstituted Peridinin-Chlorophyll a-protein, has been investigated by ODMR (Optically detected magnetic resonance), and pulse EPR spectroscopies. The properties of peridinins associated with the triplet state formation in complexes reconstituted with Chl a and Chl d have been compared to those of the main-form peridinin-chlorophyll protein (MFPCP) isolated from Amphidinium carterae. In the reconstituted samples no signals due to the presence of chlorophyll triplet states have been detected, during either steady state illumination or laser-pulse excitation. This demonstrates that reconstituted complexes conserve total quenching of chlorophyll triplet states, despite the biochemical treatment and reconstitution with the non-native Chl d pigment. Zero field splitting parameters of the peridinin triplet states are the same in the two reconstituted samples and slightly smaller than in native MFPCP. Analysis of the initial polarization of the photoinduced Electron-Spin-Echo detected spectra and their time evolution, shows that, in the reconstituted complexes, the triplet state is probably l...Continue Reading

References

Sep 12, 1996·Biochimica Et Biophysica Acta·F P SharplesR G Hiller
Mar 1, 1996·Photochemistry and Photobiology·H A Frank, R J Cogdell
Oct 12, 2000·Biophysical Journal·A DamjanovićK Schulten
Jun 19, 2002·Biochemistry·Stefano SantabarbaraDonatella Carbonera
Sep 29, 2005·Journal of Magnetic Resonance·Stefan Stoll, Arthur Schweiger
Nov 1, 2007·Photosynthesis Research·S MackowskiC Bräuchle

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Citations

Sep 18, 2010·European Journal of Cell Biology·Tim SchulteEckhard Hofmann
Jul 23, 2013·Biochimica Et Biophysica Acta·Marilena Di ValentinDonatella Carbonera
Jul 31, 2013·Progress in Lipid Research·Ildikó DomonkosBettina Ughy
Apr 2, 2011·The Journal of Physical Chemistry. B·Marcel FucimanHarry A Frank
Sep 29, 2011·The Journal of Physical Chemistry. B·Marilena Di ValentinDonatella Carbonera

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