Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells

The Journal of Reproduction and Development
Ruttachuk RungsiwiwutKamthorn Pruksananonda

Abstract

Because the diploid human embryonic stem cells (hESCs) can be successfully derived from tripronuclear zygotes thus, they can serve as an alternative source of derivation of normal karyotype hESC lines. The aim of the present study was to compare the pluripotency and trophoblast differentiation ability of hESCs derived from tripronuclear zygotes and diploid hESCs. In the present study, a total of 20 tripronuclear zygotes were cultured; 8 zygotes developed to the blastocyst stage and 1 hESC line was generated. Unlike the previous studies, chromosomal correction of tripronuclear zygotes during derivation of hESCs did not occur. The established line carries 3 sets of chromosomes and showed a numerical aberration. Although the cell line displayed an abnormal chromosome number, it was found the cell line has been shown to be pluripotent with the ability to differentiate into 3 embryonic germ layers both in vitro and in vivo. The expression of X inactive specific transcript (XIST) in mid-passage (passage 42) of undifferentiated triploid hESCs was detected, indicating X chromosome inactivation of the cell line. Moreover, when this cell line was induced to differentiate toward the trophoblast lineage, morphological and functional tropho...Continue Reading

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Citations

Sep 4, 2016·Human Reproduction Update·Teena Kjb GamageJoanna L James
Mar 27, 2018·Biology of Reproduction·R Michael RobertsYing Yang
Jan 5, 2019·Stem Cells and Development·Marjan OmidiMohammad Ali Khalili

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Methods Mentioned

BETA
PCR
ELISA
enzymatic dissociation
chromosomal aberrations
chromosomal aberration

Software Mentioned

ABI
GraphPad Prism

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