Triptolide ameliorates lipopolysaccharide-induced acute lung injury in rats

European Journal of Medical Research
Jianling GaoJianping Yang

Abstract

Acute lung injury (ALI) is a serious clinical syndrome with a high rate of mortality. In this study, the effects of triptolide on lipopolysaccharide (LPS)-induced ALI in rats were investigated. Sixty-five male Sprague Dawley rats(approved by ethics committee of the First Affiliated Hospital of Soochow University) were randomly divided into five groups. The control group was injected with 2.5 mL saline/kg body weight via the tail vein and intraperitoneally with 1% dimethyl sulfoxide (DMSO) (n = 5). The L group was administered with 0.2% LPS dissolved in saline (5 mg/kg) to induce ALI via the tail vein (n = 15). The TP1, TP2, and TP3 groups were treated as rats in the L group and then intraperitoneally injected with 25, 50, and 100 μg triptolide/kg body weight, respectively (15 rats per group). Blood samples from the left heart artery were taken for blood gas analysis at 1 hour before injection and at 1, 3, 6, and 12 hours after saline and DMSO administration in the control group, LPS injection in the L group, and triptolide injection in the TP1, TP2, and TP3 groups. Lung wet-to-dry weight (W/D) ratio, diffuse alveolar damage (DAD) score, TNF-α levels, and mRNA and protein expression of toll-like receptor 4 (TLR4) were analyzed. ...Continue Reading

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Citations

Oct 16, 2015·Immunopharmacology and Immunotoxicology·Liyu HeYouming Peng
Dec 22, 2015·International Immunopharmacology·Marwa E AbdelmageedGhada M Suddek
Jun 20, 2015·Burns : Journal of the International Society for Burn Injuries·Huiqi WangChengjin Gao
Dec 30, 2014·Cytotherapy·Somia H Abd-AllahMohammed Abu El-Magd

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Methods Mentioned

BETA
transfection
bronchoalveolar lavage
blood collection
enzyme-linked immunosorbent assay
PCR
Enzyme-linked

Software Mentioned

SAS
Primer Premier

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