Triptolide Suppresses Glomerular Mesangial Cell Proliferation in Diabetic Nephropathy Is Associated with Inhibition of PDK1/Akt/mTOR Pathway

International Journal of Biological Sciences
Fei HanLiming Chen

Abstract

Mesangial cell proliferation has been identified as a mainly contributing factor to glomerulosclerosis, which is typical of diabetic nephropathy. However, the specific mechanisms and therapies remain unclear. PDK1 is a critical regulator of cell proliferation, but the specific role of PDK1 in diabetic nephropathy has not been fully illuminated. In the current study, we demonstrated that triptolide (TP) ameliorated albuminuria in the high fat diet/STZ-induced diabetic rats. TP also suppressed the increased proliferating cell markers Ki-67 and PCNA in the kidney tissues. Our results of MTT and cell cycle analysis further confirmed that TP significantly inhibited mesangial cell proliferation, and the inhibition of PDK1/Akt/mTOR pathway might be the underlying mechanisms. In addition, we also found that the PDK1 activator (PS48) could reverse the cell proliferation inhibition role of TP. These data suggest that TP may be useful in prevention of diabetic glomerulosclerosis and that PDK1/Akt/mTOR pathway might be the underlying mechanism.

Citations

Jan 10, 2018·Frontiers in Pharmacology·Youling FanJun Zhou
Mar 8, 2018·American Journal of Physiology. Renal Physiology·Ying JingChanglin Mei
Feb 13, 2020·Frontiers in Endocrinology·Wen-Ning XuSheng-Dan Jiang
Nov 12, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guihua Fang, Bo Tang
Nov 11, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wei-Jun HuangJin-Xi Zhao
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhiwei ZhangWeifeng Mao
May 27, 2021·International Journal of Immunopathology and Pharmacology·Jihua TianYun Zhou
Jul 1, 2021·Néphrologie & thérapeutique·Yue QiuChun Zhang
Aug 7, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Vikram PatialYogendra Padwad
Jul 30, 2020·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·Xun FangHongyan Zhou

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
light microscopy
electrophoresis

Software Mentioned

SPSS
Modfit

Related Concepts

Related Feeds

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.