Tris(1,3-dichloro-2-propyl) Phosphate Exposure During the Early-Blastula Stage Alters the Normal Trajectory of Zebrafish Embryogenesis

Environmental Science & Technology
Subham DasguptaDavid C Volz

Abstract

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is an organophosphate flame retardant used around the world. Within zebrafish, we previously showed that initiation of TDCIPP exposure during cleavage (0.75 h post-fertilization, hpf) results in epiboly disruption at 6 hpf, leading to dorsalized embryos by 24 hpf, a phenotype that mimics the effects of dorsomorphin (DMP), a bone morphogenetic protein (BMP) antagonist that dorsalizes embryos in the absence of epiboly defects. The objective of this study was to (1) investigate the role of BMP signaling in TDCIPP-induced toxicity during early embryogenesis, (2) identify other pathways and processes targeted by TDCIPP, and (3) characterize the downstream impacts of early developmental defects. Using zebrafish as a model, we first identified a sensitive window for TDCIPP-induced effects following exposure initiation at 0.75 hpf. We then investigated the effects of TDCIPP on the transcriptome during the first 24 h of development using mRNA sequencing and amplicon sequencing. Finally, we relied on whole-mount immunohistochemistry, dye-based labeling, and morphological assessments to study abnormalities later in embryonic development. Overall, our data suggest that the initiation of TDCIPP...Continue Reading

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Citations

Sep 16, 2019·Environmental Science and Pollution Research International·Simin WangXueyan Li
Aug 11, 2019·Ecotoxicology and Environmental Safety·DongYoung RhyuKi-Tae Kim
Jan 31, 2020·Environment International·Aalekhya ReddamDavid C Volz
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Subham DasguptaRobyn L Tanguay
Sep 12, 2020·Ecotoxicology and Environmental Safety·Kazuki KandaHisato Iwata
Jun 25, 2021·Current Protocols·Subham DasguptaDavid C Volz
Jul 16, 2020·Chemico-biological Interactions·Paulo FernandesLuís Félix

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