Tris(2-aminoethyl)amine-based α-branched fatty acid amides - Synthesis of lipids and comparative study of transfection efficiency of their lipid formulations

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Nicole ErdmannAndreas Langner

Abstract

The synthesis of a new class of cationic lipids, tris(2-aminoethyl)amine-based α-branched fatty acid amides, is described resulting in a series of lipids with specific variations in the lipophilic as well as the hydrophilic part of the lipids. In-vitro structure/transfection relationships were established by application of complexes of these lipids with plasmid DNA (pDNA) to different cell lines. The α-branched fatty acid amide bearing two tetradecyl chains and two lysine molecules (T14diLys) in mixture with the co-lipid 1,2-di-[(9Z)-octadec-9-enoyl]-sn-glycero-3-phosphoethanolamine (DOPE) (1/2, n/n) exhibits effective pDNA transfer in three different cell lines, namely Hep-G2, A549, and COS-7. The presence of 10% serum during lipoplex incubation of the cells did not affect the transfection efficiency. Based on that, detailed investigations of the complexation of pDNA with the lipid formulation T14diLys/DOPE 1/2 (n/n) were carried out with respect to particle size and charge using dynamic light scattering (DLS), ζ-potential measurements, and transmission electron microscopy (TEM). Additionally, the lipoplex uptake was investigated by confocal laser scanning microscopy (CLSM). Overall, lipoplexes prepared from T14diLys/DOPE 1/2 ...Continue Reading

References

Nov 16, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·C M VargaD A Lauffenburger
Nov 27, 2001·Science·D Ferber
Apr 18, 2002·Biochimica Et Biophysica Acta·Inge S ZuhornDick Hoekstra
Apr 30, 2003·Applied Microbiology and Biotechnology·M Thomas, A M Klibanov
Apr 27, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Inge S ZuhornDick Hoekstra
Jul 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hongtao LvJie Yan
Oct 5, 2006·European Biophysics Journal : EBJ·Inge S ZuhornDick Hoekstra
Oct 5, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Baichao MaHongtao Lv
Nov 21, 2007·The Journal of Physical Chemistry. B·Maria N AntipinaGerald Brezesinski
Apr 22, 2008·Progress in Lipid Research·Caroline LonezJean-Marie Ruysschaert
Dec 17, 2008·Current Pharmaceutical Design·A Aigner
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Mar 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gaurav SahayAlexander V Kabanov
Mar 1, 2011·International Journal of Pharmaceutics·Christian WölkBodo Dobner
Jul 27, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zia ur RehmanInge S Zuhorn
May 29, 2012·Advanced Drug Delivery Reviews·Caroline LonezJean-Marie Ruysschaert
Sep 22, 2012·Journal of Drug Targeting·Shubiao ZhangLeaf Huang
Dec 26, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zia ur RehmanDick Hoekstra
Apr 5, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Christian WölkBodo Dobner
Aug 30, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Kristina Bram KnudsenMartin Roursgaard
Jul 2, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian WölkGerald Brezesinski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.