Triskelion structure of the Gli521 protein, involved in the gliding mechanism of Mycoplasma mobile.

Journal of Bacteriology
Takahiro NonakaMakoto Miyata

Abstract

Mycoplasma mobile binds to solid surfaces and glides smoothly and continuously by a unique mechanism. A huge protein, Gli521 (521 kDa), is involved in the gliding machinery, and it is localized in the cell neck, the base of the membrane protrusion. This protein is thought to have the role of force transmission. In this study, the Gli521 protein was purified from M. mobile cells, and its molecular shape was studied. Gel filtration analysis showed that the isolated Gli521 protein forms mainly a monomer in Tween 80-containing buffer and oligomers in Triton X-100-containing buffer. Rotary shadowing electron microscopy showed that the Gli521 monomer consisted of three parts: an oval, a rod, and a hook. The oval was 15 nm long by 11 nm wide, and the filamentous part composed of the rod and the hook was 106 nm long and 3 nm in diameter. The Gli521 molecules form a trimer, producing a "triskelion" reminiscent of eukaryotic clathrin, through association at the hook end. Image averaging of the central part of the triskelion suggested that there are stable and rigid structures. The binding site of a previously isolated monoclonal antibody on Gli521 images showed that the hook end and oval correspond to the C- and N-terminal regions, respe...Continue Reading

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Citations

Jun 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daisuke NakaneKoji Nakayama
Oct 18, 2011·Journal of Bacteriology·Daisuke Nakane, Makoto Miyata
Jun 11, 2010·Annual Review of Microbiology·Makoto Miyata
Jun 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yoshiaki KinositaTakayuki Nishizaka
Oct 27, 2015·Current Opinion in Microbiology·Makoto Miyata, Tasuku Hamaguchi
Dec 30, 2015·Current Opinion in Microbiology·Makoto Miyata, Tasuku Hamaguchi
Mar 31, 2016·Molecular Microbiology·Beiyan Nan, David R Zusman
Oct 28, 2015·Journal of Bacteriology·Hanako MorioMakoto Miyata
Aug 2, 2018·Scientific Reports·Yoshiaki KinositaTakayuki Nishizaka

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