Triterpene Acid (3 -O -p -Coumaroyltormentic Acid) Isolated From Aronia Extracts Inhibits Breast Cancer Stem Cell Formation through Downregulation of c-Myc Protein

International Journal of Molecular Sciences
Hack Sun ChoiDong-Sun Lee

Abstract

Cancer stem cells (CSCs) are drug-resistant and radiation-resistant cancer cells that are responsible for tumor progression and maintenance, cancer recurrence, and metastasis. Targeting breast CSCs with phytochemicals is a new paradigm for cancer prevention and treatment. In this study, activity-guided fractionation from mammosphere formation inhibition assays, repeated chromatographic preparations over silica gel, preparatory thin layer chromatography, and HPLC using aronia extracts led to the isolation of one compound. Using ¹H and 13C 2-dimensional nuclear magnetic resonance (NMR) as well as electrospray ionization (ESI) mass spectrometry, the isolated compound was identified as 3-O-p-coumaroyltormentic acid. This compound inhibits breast cancer cell proliferation and mammosphere formation in a dose-dependent manner and reduces the CD44high/CD24low subpopulation and aldehyde dehydrogenase (ALDH)-expressing cell population as well as the expression of the self-renewal-related genes CD44, SOX2, and OCT4.3-O-p-Coumaroyltormentic acid preferentially reduced the protein levels of c-Myc, which is a CSC survival factor, by inducing c-Myc degradation. These findings indicate the novel utilization of 3-O-p-coumaroyltormentic acid for...Continue Reading

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Citations

Jun 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Chan KoDong-Sun Lee
Apr 26, 2019·Oxidative Medicine and Cellular Longevity·Su-Lim KimDong-Sun Lee
Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ren LiuDong-Sun Lee
Nov 21, 2019·Nutrients·Clara Reglero, Guillermo Reglero
Sep 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ren LiuDong-Sun Lee
Dec 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Su-Lim KimDong-Sun Lee

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Methods Mentioned

BETA
NMR
xenograft
column chromatography
scraping
ELISA
FACS
PCR

Software Mentioned

NICE
GraphPad Prism

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