Triterpene hydrocarbon production engineered into a metabolically versatile host--Rhodobacter capsulatus

Biotechnology and Bioengineering
Nymul E KhanWayne R Curtis

Abstract

Triterpene hydrocarbon biosynthesis of the ancient algae Botryococcus braunii was installed into Rhodobacter capsulatus to explore the production of C30 hydrocarbon in a host capable of diverse growth habits-utilizing carbohydrate, sunlight or hydrogen (with CO2 fixation) as alternative energy feedstocks. Engineering an enhanced MEP pathway was also used to augment triterpene accumulation. Despite dramatically different sources of carbon and reducing power, nearly the same level of botryococcene or squalene (∼5 mg oil/g-dry-weight [gDW]) was achieved in small-scale aerobic heterotrophic, anaerobic photoheterotrophic, and aerobic chemoautotrophic growth conditions. A glucose fed-batch bioreactor reached 40 mg botryococcene/L (∼12 mg/gDW), while autotrophic bioreactor performance with CO2 , H2 , and O2 reached 110 mg/L (16.7 mg/gDW) during batch and 60 mg/L (23 mg/gDW) during continuous operation at a dilution rate corresponding to about 10% of μ(max). Batch and continuous autotrophic specific productivity was found to reach 0.5 and 0.32 mg triterpene/g DW/h, comparable to prior reports for terpene production driven by heterotrophic growth conditions. This demonstrates the feasibility of alternative feedstocks and trophic modes t...Continue Reading

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Citations

Oct 19, 2017·Nucleic Acids Research·Karel MiettinenAlain Goossens
Jul 7, 2018·Plant Biotechnology Journal·Chase KempinskiJoe Chappell
May 10, 2019·Critical Reviews in Biotechnology·Wentao SunChun Li
Jun 13, 2019·Journal of Industrial Microbiology & Biotechnology·Enrico OrsiRuud A Weusthuis
May 28, 2019·Applied Microbiology and Biotechnology·Sandra Moser, Harald Pichler
Oct 11, 2020·Biotechnology and Bioengineering·Enrico OrsiRuud A Weusthuis
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Sheida StephensD Grant Allen
Apr 4, 2017·Current Opinion in Biotechnology·Indrani BanerjeeScott Banta
Mar 23, 2021·Frontiers in Bioengineering and Biotechnology·Haijie XueChun Li
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Oct 12, 2017·ACS Synthetic Biology·Ammara KhalidShunji Takahashi

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