Triterpenoid profiling and functional characterization of the initial genes involved in isoprenoid biosynthesis in neem (Azadirachta indica)

BMC Plant Biology
Avinash PandrekaHirekodathakallu V Thulasiram

Abstract

Neem tree (Azadirachta indica) is one of the richest sources of skeletally diverse triterpenoids and they are well-known for their broad-spectrum pharmacological and insecticidal properties. However, the abundance of Neem triterpenoids varies among the tissues. Here, we delineate quantitative profiling of fifteen major triterpenoids across various tissues including developmental stages of kernel and pericarp, flower, leaf, stem and bark using UPLC-ESI(+)-HRMS based profiling. Transcriptome analysis was used to identify the initial genes involved in isoprenoid biosynthesis. Based on transcriptome analysis, two short-chain prenyltransferases and squalene synthase (AiSQS) were cloned and functionally characterized. Quantitative profiling revealed differential abundance of both total and individual triterpenoid content across various tissues. RNA from tissues with high triterpenoid content (fruit, flower and leaf) were pooled to generate 79.08 million paired-end reads using Illumina GA ΙΙ platform. 41,140 transcripts were generated by d e novo assembly. Transcriptome annotation led to the identification of the putative genes involved in isoprenoid biosynthesis. Two short-chain prenyltransferases, geranyl diphosphate synthase (AiGDS...Continue Reading

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Citations

Aug 3, 2019·Proceedings of the National Academy of Sciences of the United States of America·Hannah HodgsonAnne Osbourn
Jun 5, 2020·BMC Plant Biology·Pan ZhangXiaochun Zhao
Apr 7, 2020·Functional Plant Biology : FPB·Mengyu LiuXiaochun Zhao
Jan 7, 2019·Food Chemistry·Ibrahim M Abu-ReidahAntonio Segura-Carretero
Feb 2, 2021·Applied Biochemistry and Biotechnology·Raja RajakaniVikrant Gupta
Feb 2, 2021·Phytochemistry·Avinash PandrekaHirekodathakallu V Thulasiram

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Datasets Mentioned

BETA
KM108315
AGV01244.1
JQ327160
ACN69082
AGB05603
BAH23428
BAA13084.1
SRR2145149

Methods Mentioned

BETA
PCR
column chromatography
affinity
Chip
RNA-seq

Software Mentioned

Velvet
Blastx
KAAS ( KEGG Automatic Annotation Server
MEGA
ClustalW
Thermo Xcalibur
TMHMM
Illumina GA [UNK] analyzer
Oases
VirtualRibosome

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