PMID: 9550052Apr 29, 1998Paper

Tritiated thymidine uptake in periodontal ligament cells of dogs' teeth stored in various media for extended time periods

Endodontics & Dental Traumatology
J G HuppI Aukhil

Abstract

The purpose of this study was to evaluate a new storage solution, Conditioned Medium, vs Hank's balanced salt solution and ViaSpan with respect to the viability of periodontal ligament cells of exarticulated teeth. Teeth were stored for periods of 6, 48, and 96 hours in Hank's balanced salt solution, ViaSpan (Dupont Pharmaceuticals), or Conditioned Medium. Teeth were cultured for 24 hours in Dulbecco's medium supplemented with tritiated thymidine. The cultured teeth were sectioned and evaluated with autoradiography. Control teeth were extracted and immediately treated as above without storage. Mitotic activity was indicated by clusters of five or more grains over the nuclei of fibroblasts in the remaining periodontal membrane. The ratio of labeled to unlabeled cells (labeling index) was calculated for each treatment group. When storage time was compared across all groups, 96 hours was significantly different from 6 and 48 hours (P < 0.001 and P < 0.012 respectively). Storage time of 6 hours was not significantly different from 48 hours (P > 0.10). After comparison of the nine experimental groups with the control group, only Hank's balanced salt solution at 96 hours was significantly different (P < 0.004).

References

Oct 1, 1992·Endodontics & Dental Traumatology·M Trope, S Friedman
Feb 1, 1986·International Journal of Oral and Maxillofacial Surgery·O Schwartz
Jan 1, 1980·Journal of Periodontal Research·T R GouldD M Brunette
Aug 1, 1995·Current Opinion in Genetics & Development·T P Yamaguchi, J Rossant
Feb 1, 1993·Journal of Periodontology·T W OatesD L Cochran

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Citations

Jun 20, 2001·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·M Trope
Jun 20, 2001·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Y PohlH Kirschner
Aug 30, 2008·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Cornelia FilippiYango Pohl
Aug 27, 2010·Dental Traumatology : Official Publication of International Association for Dental Traumatology·E CaglarB Kargul
Dec 6, 2012·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Christopher I UdoyePaul V Abbott
Jul 31, 2001·Dental Traumatology : Official Publication of International Association for Dental Traumatology·M AshkenaziH Sarnat
May 29, 2004·Journal of Endodontics·Won-Gyun ChungJin Kim
Aug 15, 2020·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Ling-Li JiXu Chen
Mar 5, 2004·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Emmanouil SigalasLynne A Opperman

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