Tritiated water exposure disrupts myofibril structure and induces mis-regulation of eye opacity and DNA repair genes in zebrafish early life stages

Aquatic Toxicology
Caroline ArcanjoBéatrice Gagnaire

Abstract

Tritium (3H) is a radioactive isotope of hydrogen. In the environment, the most common form of tritium is tritiated water (HTO). The present study aimed to identify early biomarkers of HTO contamination through the use of an aquatic model, the zebrafish (Danio rerio). We used the zebrafish embryo-larvae model to investigate the modes of action of HTO exposure at dose rates of 0.4 and 4 mGy/h, dose rates expected to induce deleterious effects on fish. Zebrafish were exposed to HTO from 3 hpf (hours post fertilization) to 96 hpf. The transcriptomic effects were investigated 24 h and 96 h after the beginning of the contamination, using mRNAseq. Results suggested an impact of HTO contamination, regardless of the dose rate, on genes involved in muscle contraction (tnnt2d, tnni2a.4, slc6a1a or atp2a1l) and eye opacity (crygm2d9, crygmxl1, mipb or lim2.3) after 24 h of contamination. Interestingly, an opposite differential expression was highlighted in genes playing a role in muscle contraction and eye opacity in 24 hpf embryos when comparing dose rates, suggesting an onset of DNA protective mechanisms. The expression of h2afx and ddb2 involved in DNA repair was enhanced in response to HTO exposure. The entrainment of circadian clock ...Continue Reading

Citations

Dec 21, 2019·Environmental Toxicology and Chemistry·Béatrice GagnaireChristelle Adam-Guillermin
Oct 3, 2019·The Review of Scientific Instruments·Jun Woo BaeSangJune Jeon
Jan 12, 2021·Journal of Hazardous Materials·Beatrice GagnaireChristelle Adam-Guillermin
Jun 26, 2020·Aquatic Toxicology·Lianguo Chen
Mar 9, 2019·Journal of Environmental Radioactivity·Caroline ArcanjoBéatrice Gagnaire

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