Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop

The EMBO Journal
L PintardB Lapeyre

Abstract

The genome of Saccharomyces cerevisiae encodes three close homologues of the Escherichia coli 2'-O-rRNA methyltransferase FtsJ/RrmJ, designated Trm7p, Spb1p and Mrm2p. We present evidence that Trm7p methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro. In a trm7Delta strain, which is viable but grows slowly, translation is impaired, thus indicating that these tRNA modifications could be important for translation efficiency. We discuss the emergence of a family of three 2'-O-RNA methyltransferases in Eukaryota and one in Prokaryota from a common ancestor. We propose that each eukaryotic enzyme is located in a different cell compartment, in which it would methylate a different RNA that can adopt a very similar secondary structure.

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Citations

Sep 17, 2005·Biochimie·Béatrice Clouet-d'OrvalAnnie Mougin
Mar 17, 2004·Journal of Molecular Biology·Mark Helm, Giuseppe Attardi
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takayuki Ohira, Tsutomu Suzuki
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