tRNA-dependent pre-transfer editing by prokaryotic leucyl-tRNA synthetase.

The Journal of Biological Chemistry
Min TanEn-Duo Wang

Abstract

To prevent genetic code ambiguity due to misincorporation of amino acids into proteins, aminoacyl-tRNA synthetases have evolved editing activities to eliminate intermediate or final non-cognate products. In this work we studied the different editing pathways of class Ia leucyl-tRNA synthetase (LeuRS). Different mutations and experimental conditions were used to decipher the editing mechanism, including the recently developed compound AN2690 that targets the post-transfer editing site of LeuRS. The study emphasizes the crucial importance of tRNA for the pre- and post-transfer editing catalysis. Both reactions have comparable efficiencies in prokaryotic Aquifex aeolicus and Escherichia coli LeuRSs, although the E. coli enzyme favors post-transfer editing, whereas the A. aeolicus enzyme favors pre-transfer editing. Our results also indicate that the entry of the CCA-acceptor end of tRNA in the editing domain is strictly required for tRNA-dependent pre-transfer editing. Surprisingly, this editing reaction was resistant to AN2690, which inactivates the enzyme by forming a covalent adduct with tRNA(Leu) in the post-transfer editing site. Taken together, these data suggest that the binding of tRNA in the post-transfer editing conforma...Continue Reading

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Citations

Nov 29, 2013·Nucleic Acids Research·Bin ZhuCharles C Richardson
Jul 22, 2011·Nucleic Acids Research·Xiao-Long ZhouEn-Duo Wang
Sep 16, 2014·FEBS Letters·Adil MoghalMichael Ibba
Aug 29, 2013·Science China. Life Sciences·Xiaolong Zhou, Enduo Wang
Oct 8, 2016·Methods : a Companion to Methods in Enzymology·Nevena Cvetesic, Ita Gruic-Sovulj
May 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bin ZhuCharles C Richardson
Nov 17, 2010·Nature Reviews. Microbiology·Noah M ReynoldsMichael Ibba
May 26, 2010·The Journal of Biological Chemistry·Morana DulicIta Gruic-Sovulj
Aug 15, 2015·The Journal of Biological Chemistry·Qing YeEn-Duo Wang
Aug 21, 2016·The Journal of Biological Chemistry·Xiao-Long ZhouEn-Duo Wang
Apr 6, 2019·Nucleic Acids Research·Qi-Yu ZengXiao-Long Zhou
May 28, 2010·The Journal of Biological Chemistry·Anand Minajigi, Christopher S Francklyn
Apr 30, 2017·The Journal of Biological Chemistry·Qing YeEn-Duo Wang
Nov 1, 2012·EcoSal Plus·Richard Giegé, Mathias Springer

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