Abstract
Idiopathic nephrotic syndrome (INS) is a disease involving injury to podocytes in the glomerular filtration barrier, and its specific causes have not been elucidated. Transfer RNA-derived fragments (tRFs), products of precise tRNA cleavage, have been indicated to play critical roles in various diseases. Currently, there is no relevant research on the role of tRFs in INS. This study intends to explore the changes in and importance of tRFs during podocyte injury in vitro and to further analyze the potential mechanism of INS. Differentially expressed tRFs in the adriamycin-treated group were identified by high-throughput sequencing and further verified by quantitative RT-PCR. In total, 203 tRFs with significant differential expression were identified, namely, 102 upregulated tRFs and 101 downregulated tRFs (q < 0.05, ∣log2FC | ≥2). In particular, AS-tDR-008924, AS-tDR-011690, tDR-003634, AS-tDR-013354, tDR-011031, AS-tDR-001008, and AS-tDR-007319 were predicted to be involved in podocyte injury by targeting the Gpr, Wnt, Rac1, and other genes. Furthermore, gene ontology analysis showed that these differential tRFs were strongly associated with podocyte injury processes such as protein binding, cell adhesion, synapses, the actin cy...Continue Reading
References
May 11, 2005·Kidney International·Takeshi NakatsueHiroshi Kawachi
Apr 26, 2007·Kidney International·S J ShanklandP Mundel
Sep 7, 2007·Trends in Cell Biology·Christian FaulPeter Mundel
Sep 12, 2008·American Journal of Physiology. Renal Physiology·Jeffrey W PippinStuart J Shankland
Jul 25, 2009·Journal of the American Society of Nephrology : JASN·Chunsun DaiYouhua Liu
Nov 12, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Marie Frimodt-MøllerAnne-Lise Kamper
Nov 26, 2009·Genes & Development·Yong Sun LeeAnindya Dutta
Apr 9, 2010·Kidney International·Chunsun DaiYouhua Liu
Jan 18, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ilene BoucherBradley M Denker
Oct 27, 2012·Journal of the American Society of Nephrology : JASN·Piero RuggenentiGiuseppe Remuzzi
Nov 30, 2012·Annual Review of Medicine·Jochen Reiser, Sanja Sever
Jan 23, 2013·Nature Reviews. Nephrology·Norman Sachs, Arnoud Sonnenberg
Apr 24, 2013·Nature Reviews. Nephrology·Paul Thomas BrinkkoetterThomas Benzing
Feb 18, 2014·The Journal of Clinical Investigation·Xuefei TianShuta Ishibe
Jul 12, 2014·Journal of the American Society of Nephrology : JASN·Lili ZhouYouhua Liu
Sep 16, 2014·FEBS Letters·Paul Anderson, Pavel Ivanov
Nov 19, 2014·The Journal of Biological Chemistry·Benoit DenhezPedro Geraldes
Jan 30, 2015·The Journal of Pharmacology and Experimental Therapeutics·Frank Park
Mar 31, 2015·World Journal of Pediatrics : WJP·Kazunari KanekoShogo Kato
Jun 7, 2015·Cellular Signalling·Zhou JingZhu-ge Yi-Feng
Jun 10, 2015·Nature Reviews. Nephrology·Lili Zhou, Youhua Liu
Sep 24, 2015·Molecular Nutrition & Food Research·Xiao-Qin DingLing-Dong Kong
Jan 23, 2016·American Journal of Physiology. Renal Physiology·Xiaoyang WanWeibin Zhou
May 28, 2016·Cell Cycle·Manuel HagenChristoph Daniel
Jun 6, 2016·Trends in Biochemical Sciences·Pankaj KumarAnindya Dutta
Aug 31, 2016·Nature Reviews. Nephrology·Luca PericoGiuseppe Remuzzi
Jul 1, 2017·American Journal of Physiology. Renal Physiology·Yanggang YuanChangying Xing
Mar 14, 2018·American Journal of Nephrology·Janice Crespo-SalgadoDiego H Aviles
May 12, 2018·Genes·Siqi LiJinghao Sheng
Jun 19, 2018·Lancet·Damien G NooneRulan Parekh
Jul 1, 2018·Nature Reviews. Nephrology·Rubina NovelliGiuseppe Remuzzi
Jul 3, 2018·Science Advances·Kathryn E HaleyPaul A Reynolds
Oct 23, 2018·International Journal of Rheumatic Diseases·Yan ZhangXiaodong Liu
Oct 27, 2018·Journal of Cellular Physiology·Yangyang CuiHui Xie
Feb 26, 2019·Journal of Cellular and Molecular Medicine·Hui ChenDeliang Liu
Sep 20, 2019·Journal of Cellular Physiology·Yue HuangHui Xie
Sep 29, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maurizio FalconiAugusto Amici
Oct 21, 2019·Biochemical and Biophysical Research Communications·Huimin ShiAiqing Zhang