tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner.

RNA
Canan KuscuAnindya Dutta

Abstract

tRNA related RNA fragments (tRFs), also known as tRNA-derived RNAs (tdRNAs), are abundant small RNAs reported to be associated with Argonaute proteins, yet their function is unclear. We show that endogenous 18 nucleotide tRFs derived from the 3' ends of tRNAs (tRF-3) post-transcriptionally repress genes in HEK293T cells in culture. tRF-3 levels increase upon parental tRNA overexpression. This represses target genes with a sequence complementary to the tRF-3 in the 3' UTR. The tRF-3-mediated repression is Dicer-independent, Argonaute-dependent, and the targets are recognized by sequence complementarity. Furthermore, tRF-3:target mRNA pairs in the RNA induced silencing complex associate with GW182 proteins, known to repress translation and promote the degradation of target mRNAs. RNA-seq demonstrates that endogenous target genes are specifically decreased upon tRF-3 induction. Therefore, Dicer-independent tRF-3s, generated upon tRNA overexpression, repress genes post-transcriptionally through an Argonaute-GW182 containing RISC via sequence matches with target mRNAs.

References

Jan 17, 2003·Nature·Natividad Gomez-RomanRobert J White
Feb 22, 2005·Nature Cell Biology·Azadeh ArabiAnthony P H Wright
Feb 22, 2005·PLoS Biology·Julius BrenneckeStephen M Cohen
Jun 24, 2005·Nature·Thimmaiah P ChendrimadaRamin Shiekhattar
Nov 8, 2005·Cell·Richard I GregoryRamin Shiekhattar
Dec 21, 2005·Current Opinion in Structural Biology·Yong Xiong, Thomas A Steitz
Jul 7, 2007·Molecular Cell·Andrew GrimsonDavid P Bartel
Sep 18, 2007·RNA·Cydney B NielsenChristopher B Burge
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Mar 18, 2008·Nature Structural & Molecular Biology·Ana EulalioElisa Izaurralde
Sep 29, 2009·Nucleic Acids Research·Mariana Pavon-EternodTao Pan
Nov 26, 2009·Genes & Development·Yong Sun LeeAnindya Dutta
Apr 29, 2010·Nature·Sihem CheloufiGregory J Hannon
Nov 23, 2011·RNA·Juan G Betancur, Yukihide Tomari
Oct 2, 2012·Cell·Charles Y LinRichard A Young
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Roy L MauteRiccardo Dalla-Favera
Jun 5, 2013·Nature Reviews. Genetics·Gunter Meister
Sep 2, 2014·Nature Reviews. Cancer·William D FoulkesThomas F Duchaine
Nov 14, 2014·Nucleic Acids Research·Pankaj KumarAnindya Dutta
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jun 17, 2015·Nature Reviews. Genetics·Stefanie Jonas, Elisa Izaurralde
Jul 28, 2015·Nature Methods·Guanqun ZhengTao Pan
Mar 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Young-Kook KimV Narry Kim
Apr 5, 2016·Nature Biotechnology·Nicolas L BrayLior Pachter
May 14, 2016·Nature Communications·Thomas B HansenJørgen Kjems
Jun 6, 2016·Trends in Biochemical Sciences·Pankaj KumarAnindya Dutta
Jul 1, 2017·Cell·Andrea J SchornRob Martienssen
Nov 18, 2017·Plant & Cell Physiology·Agnieszka ThompsonWojciech M Karlowski

❮ Previous
Next ❯

Citations

Jan 4, 2019·Nature Cell Biology·Marcos Francisco Perez, Ben Lehner
Feb 20, 2019·Non-coding RNA·Shengqian DouJian Lu
Apr 27, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vivek M Advani, Pavel Ivanov
Jul 10, 2019·Pigment Cell & Melanoma Research·Eric LondinIsidore Rigoutsos
Nov 19, 2019·Database : the Journal of Biological Databases and Curation·Alessandro La FerlitaAlfredo Pulvirenti
Dec 14, 2019·Genes & Development·Ana BoskovicOliver J Rando
Sep 29, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maurizio FalconiAugusto Amici
Jun 7, 2020·International Journal of Molecular Sciences·Vasiliki I PantazopoulouEma Anastasiadou
Jul 21, 2020·Scientific Reports·Nicolas BonhoureNouria Hernandez
Oct 16, 2019·RNA Biology·Lingyu GuanAndrey Grigoriev
Sep 6, 2020·Nucleic Acids Research·Rogan Magee, Isidore Rigoutsos
Sep 10, 2020·RNA Biology·Matthew D Berg, Christopher J Brandl
Sep 21, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yijing ShenJunming Guo
Oct 5, 2019·The Journal of Biological Chemistry·Zhangli SuAnindya Dutta
Oct 1, 2020·Cancers·Joonhyeong ParkSuhwan Chang
Dec 20, 2018·Epigenomics·Katerina GrafanakiConstantinos Stathopoulos
Nov 2, 2019·Journal of Cellular Biochemistry·Zhongliang MaYanli Li
Nov 23, 2019·Cells·Christiaan J Stavast, Stefan J Erkeland
Jul 2, 2020·Signal Transduction and Targeted Therapy·Yaoyao XieJunming Guo
Sep 6, 2020·Journal of Hematology & Oncology·Mengqian YuYan Lu
Nov 30, 2019·International Journal of Molecular Sciences·Preethi KrishnanCarmella Evans-Molina
Mar 19, 2020·RNA Biology·Domenico RosaceSandra Blanco
Aug 20, 2020·Circulation. Genomic and Precision Medicine·Saumya DasUNKNOWN American Heart Association Council on Genomic and Precision Medicine; Council on Arteriosclerosis, Thrombosis and Vascular B
May 15, 2019·Annual Review of Genomics and Human Genetics·Ashleigh E SchafferJeffery M Coller
Feb 11, 2020·Frontiers in Cell and Developmental Biology·Madlen MüllerConstance Ciaudo
Sep 16, 2020·Pharmacological Reviews·Ai-Ming YuMei-Juan Tu
May 28, 2020·Frontiers in Genetics·Ruaidhri CappaAmy J McKnight
Jul 29, 2020·Viruses·Harrison Cullen, Andrea J Schorn
Oct 1, 2020·International Journal of Molecular Sciences·Dylan J Kiltschewskij, Murray J Cairns
Jul 1, 2020·BMC Plant Biology·Bhavika TiwariWolfgang Frank
Oct 10, 2020·Nucleic Acids Research·Ningshan LiZuoheng Wang
Oct 13, 2020·Frontiers in Pharmacology·Jun CaoDa-Zhi Wang
Sep 23, 2020·The Journal of Infectious Diseases·Monika M LooneyMarc K Halushka
Nov 17, 2020·Frontiers in Genetics·Anahi Molla-HermanJean-René Huynh
Dec 15, 2020·Current Opinion in Organ Transplantation·Thomas RousselleValeria R Mas
Apr 19, 2019·Cancer Research·Aristeidis G TelonisIsidore Rigoutsos
Jan 5, 2021·Frontiers in Molecular Biosciences·Irem Avcilar-Kucukgoze, Anna Kashina

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.