tRNA recognition by CCA-adding enzyme

Nucleic Acids Research. Supplement
Yukihide TomariT Ueda


The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC.] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.


Apr 5, 2017·Biomolecules·Christian LorenzMario Mörl
Jan 16, 2020·International Journal of Molecular Sciences·Lieselotte ErberMario Mörl
Nov 17, 2020·Frontiers in Microbiology·Natalie KrahnDieter Söll
Dec 3, 2020·International Journal of Molecular Sciences·Oliver HennigMario Mörl

Related Concepts

CCA-adding enzyme, human
RNA Conformation
RNA Nucleotidyltransferases
Transfer RNA
Substrate Specificity
Caenorhabditis elegans

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