PMID: 7030739Oct 1, 1981Paper

tRNA thiolated pyrimidines as targets for near-ultraviolet-induced synthesis of guanosine tetraphosphate in Escherichia coli

European Journal of Biochemistry
G ThomasA Favre

Abstract

Illumination with near-ultraviolet light triggers synthesis of ppGpp (guanosine 3'-diphosphate 5'-diphosphate) not only in growing Escherichia coli cells containing the putative chromophore 4-thiouridine in their tRNAs [Ramabhadran, T. V and Jagger, J. (1976) Proc. Natl Acad. Sci. USA, 73, 59--69], but also in nuv- cells which lack 4-thiouridine. The burst of ppGpp in nuv- cells is, however, induced exclusively by light of wavelengths shorter than 350 nm. Its maximum level is half that obtained in the parental strain. This ppGpp synthesis is also under the control of the relA gene, indicating that it is due to the accumulation of uncharged tRNAs. A candidate likely to trigger this effect is a 5-methylaminomethyl-2-thiouracil residues present in the first position of the anticodon loop of tRNAGlu, tRNALys and one tRNAGln isoacceptor. In conditions in vitro, this base is highly photoreactive at wavelengths shorter than 350 nm. Furthermore, near-ultraviolet-photomodified tRNAGlu and tRNALys become poor substrates of their acylation enzyme.

References

Dec 1, 1977·Photochemistry and Photobiology·G Thomas
Mar 1, 1979·Nucleic Acids Research·D GhosalH Saedler
Jan 1, 1979·Nucleic Acids Research·A C LuiM Smith
Apr 5, 1976·Biochemical and Biophysical Research Communications·M Uzan, A Danchin
Oct 1, 1976·Photochemistry and Photobiology·J P BalliniA Favre
Oct 27, 1975·Biochemical and Biophysical Research Communications·G Thomas, A Favre
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T V Ramabhadran, J Jagger
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W A Haseltine, R Block
Dec 1, 1980·European Journal of Biochemistry·G Thomas, A Favre
Aug 1, 1972·FEBS Letters·Alain FavreJean Louis Fourrey

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Citations

Sep 1, 1987·Journal of Photochemistry and Photobiology. B, Biology·A Caldeira de Araujo, A Favre
Jan 7, 2004·Journal of Photochemistry and Photobiology. B, Biology·Oscar J Oppezzo, Ramón A Pizarro
May 11, 1992·Nucleic Acids Research·K TamuraM Shimizu
Jul 3, 2007·Nucleic Acids Research·Daniel KlaimanGabriel Kaufmann
Mar 15, 1984·European Journal of Biochemistry·A BlanchetotA Favre
Feb 1, 1985·European Journal of Biochemistry·A Caldeira de Araujo, A Favre
Nov 1, 1986·Photochemistry and Photobiology·H LangF Bergter
Feb 1, 1986·Photochemistry and Photobiology·E Hajnsdorf, A Favre
Jan 1, 1983·Zeitschrift für allgemeine Mikrobiologie·K Riedel
Aug 14, 2020·Genes·Ashley M EdwardsPatricia C Dos Santos
May 1, 2014·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Nov 1, 2005·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Feb 15, 1988·Biochemical and Biophysical Research Communications·M O Blondel, A Favre

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