tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases.

RNA
Jane E Jackman, Eric M Phizicky

Abstract

All eukaryotic tRNA(His) molecules are unique among tRNA species because they require addition of a guanine nucleotide at the -1 position by tRNA(His) guanylyltransferase, encoded in yeast by THG1. This G(-1) residue is both necessary and sufficient for aminoacylation of tRNA by histidyl-tRNA synthetase in vitro and is required for aminoacylation in vivo. Although Thg1 is presumed to be highly specific for tRNA(His) to prevent misacylation of tRNAs, the source of this specificity is unknown. We show here that Thg1 is >10,000-fold more selective for its cognate substrate tRNA(His) than for the noncognate substrate tRNA(Phe). We also demonstrate that the GUG anticodon of tRNA(His) is a crucial Thg1 identity element, since alteration of this anticodon in tRNA(His) completely abrogates Thg1 activity, and the simple introduction of this GUG anticodon to any of three noncognate tRNAs results in significant Thg1 activity. For tRNA(Phe), k(cat)/K(M) is improved by at least 200-fold. Thg1 is the only protein other than aminoacyl-tRNA synthetases that is known to use the anticodon as an identity element to discriminate among tRNA species while acting at a remote site on the tRNA, an unexpected link given the lack of any identifiable sequ...Continue Reading

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Citations

Dec 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Akiyoshi NakamuraMin Yao
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Maria G AbadJane E Jackman
Jul 28, 2010·Nucleic Acids Research·Antonio PlacidoLaurence Maréchal-Drouard
Sep 3, 2010·Genes & Development·Eric M Phizicky, Anita K Hopper
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samantha J HydeSylvie Doublié
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·John J Perona, Javin P Oza
Sep 6, 2011·Nucleic Acids Research·Ilka U HeinemannDieter Söll
Dec 18, 2012·Nucleic Acids Research·Bhalchandra S RaoJane E Jackman
Nov 21, 2012·Current Opinion in Nephrology and Hypertension·Madeline MurphyCatherine Godson
Nov 26, 2009·FEBS Letters·Eric M Phizicky, Juan D Alfonzo
Jul 24, 2010·FEBS Letters·Ilka U HeinemannDieter Söll
Oct 26, 2011·Seminars in Cell & Developmental Biology·Zdeněk ParisJuan D Alfonzo
Feb 28, 2015·Nucleic Acids Research·Qingnan TianWei Xie
Aug 4, 2016·Nucleic Acids Research·Yicheng LongJane E Jackman
Jun 30, 2018·RNA·Jinjie ShangYing Huang
Apr 17, 2014·The Journal of Biological Chemistry·Maria G AbadJane E Jackman
Sep 6, 2020·Nucleic Acids Research·Rogan Magee, Isidore Rigoutsos
Sep 10, 2020·RNA Biology·Matthew D Berg, Christopher J Brandl
Aug 22, 2018·Frontiers in Genetics·Kozo Tomita, Yining Liu
Mar 22, 2019·International Journal of Molecular Sciences·Marie-Theres PöhlerMario Mörl
Apr 18, 2020·Wiley Interdisciplinary Reviews. RNA·Phillip J McCownJessica A Brown
Aug 11, 2018·Biochemical and Biophysical Research Communications·Akiyoshi NakamuraYasuo Komatsu
May 22, 2021·Royal Society Open Science·Jana WiedermannováYulia Yuzenkova

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