Troglitazone induced apoptosis of human pterygium fibroblasts through a mitochondrial-dependent pathway.

International Journal of Ophthalmology
Xiao-Xi YangJin-Tang Xu

Abstract

To study the effect of troglitazone on primary culture human pterygium fibroblasts (HPF). Cell viability loss and apoptosis were quantified by cell counting kit-8, AnnexinV-FITC/PI double staining, caspases activity test and western blotting. Flow cytometry was used to detect mitochondrial membrane potential. Peroxisome proliferator-activated receptor γ (PPAR-γ) was positively expressed in pterygium specimens (n=5). Troglitazone showed dose-dependent inhibition of cell survival, induced phospholipids redistribution, activated caspase-3, -9, and altered mitochondrial potential. Western blot assay demonstrated the increase of Bax/Bcl-2 protein ratio. Troglitazone induced apoptosis of HPF through a mitochondrial-dependent pathway.

Citations

Jul 7, 2015·Journal of Ophthalmology·Su ZhangNan Hu

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