Trogocytosis-associated cell to cell spread of intracellular bacterial pathogens

ELife
Shaun SteeleThomas Kawula

Abstract

Macrophages are myeloid-derived phagocytic cells and one of the first immune cell types to respond to microbial infections. However, a number of bacterial pathogens are resistant to the antimicrobial activities of macrophages and can grow within these cells. Macrophages have other immune surveillance roles including the acquisition of cytosolic components from multiple types of cells. We hypothesized that intracellular pathogens that can replicate within macrophages could also exploit cytosolic transfer to facilitate bacterial spread. We found that viable Francisella tularensis, as well as Salmonella enterica bacteria transferred from infected cells to uninfected macrophages along with other cytosolic material through a transient, contact dependent mechanism. Bacterial transfer occurred when the host cells exchanged plasma membrane proteins and cytosol via a trogocytosis related process leaving both donor and recipient cells intact and viable. Trogocytosis was strongly associated with infection in mice, suggesting that direct bacterial transfer occurs by this process in vivo.

References

Dec 1, 1990·The Journal of Cell Biology·L G TilneyD A Portnoy
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria Carmen Ramirez, Luis J Sigal
Feb 18, 2003·Science·Tadahiko IgakuraCharles R M Bangham
Aug 28, 2003·Nature Immunology·Etienne Joly, Denis Hudrisier
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brian P DolanSuzanne Ostrand-Rosenberg
Sep 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Claire ChecrounJean Celli
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lesley Ann SmythRobert Lechler
Oct 6, 2010·Infection and Immunity·Brittany L MortensenThomas H Kawula
Nov 17, 2010·European Journal of Immunology·Kerstin RosenitsPeter Aichele
Dec 15, 2010·American Journal of Physiology. Cell Physiology·G KanaporisV Valiunas
Feb 7, 2012·Cellular & Molecular Immunology·Peng GuLi Zhang
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lijin LiWilliam E Gillanders
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Oct 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Douglas G Osborne, Scott A Wetzel
Nov 5, 2013·Physiology·Robert S Rogers, Jahar Bhattacharya
Dec 24, 2013·Current Biology : CB·Shawna C O ReedMatthew D Welch
Apr 2, 2014·Infection and Immunity·Lydia M RobertsJeffrey A Frelinger
May 20, 2015·Infection and Immunity·J BruntonT Kawula

❮ Previous
Next ❯

Citations

Jul 28, 2016·Seminars in Cell & Developmental Biology·Javier Pizarro-CerdáPascale Cossart
Oct 25, 2016·Frontiers in Immunology·Aranzazu Cruz-Adalia, Esteban Veiga
Feb 27, 2016·ELife·Emilie Bourdonnay, Thomas Henry
Mar 17, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Quigly DragotakesArturo Casadevall
Sep 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Amber Dance
May 6, 2020·Infection and Immunity·Akhila BettadapurKatherine S Ralston
Apr 1, 2017·Frontiers in Immunology·Daniel A Powell, Jeffrey A Frelinger
Apr 13, 2017·Frontiers in Cellular and Infection Microbiology·Jason ZiveriAlain Charbit
Jul 25, 2019·Frontiers in Cellular and Infection Microbiology·Klara Kubelkova, Ales Macela
Feb 7, 2018·PLoS Biology·Frances MercerPatricia J Johnson
Nov 1, 2017·Frontiers in Cellular and Infection Microbiology·Zuzana KrocovaKlara Kubelkova
May 10, 2018·Frontiers in Cellular and Infection Microbiology·Daniel L ClemensMarcus A Horwitz
Oct 30, 2020·Bio-protocol·Sedelia R DominguezShaun P Steele
Sep 27, 2020·Journal of Translational Medicine·Hesam Saghaei BagheriEmel Sokullu
Jun 3, 2021·Cells·Masafumi NakayamaShin-Ichiro Yamaguchi
Jun 3, 2021·Cells·Kensuke Miyake, Hajime Karasuyama
Jun 19, 2021·Critical Reviews in Biochemistry and Molecular Biology·Zuzana NahackaJiri Neuzil
Jul 3, 2021·Cells·Jim ReedScott A Wetzel
Jul 10, 2021·Annual Review of Cell and Developmental Biology·John P McCutcheon

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
FACS
Fluorescence
protein exchange
transgenic
fluorescence microscopy
X-ray

Software Mentioned

ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved