Trojan horse monocyte-mediated delivery of conjugated polymer nanoparticles for improved photodynamic therapy of glioblastoma.

Nanomedicine
Luis Exequiel IbarraRodrigo Emiliano Palacios

Abstract

Aim: To assess monocyte-based delivery of conjugated polymer nanoparticles (CPNs) for improved photodynamic therapy (PDT) in glioblastoma (GBM). Materials & methods: Human monocyte cells (THP-1) and murine monocytes isolated from bone marrow (mBMDMs) were employed as stealth CPN carriers to penetrate into GBM spheroids and an orthotopic model of the tumor. The success of PDT, using this cell-mediated targeting strategy, was determined by its effect on the spheroids. Results: CPNs did not affect monocyte viability in the absence of light and did not show nonspecific release after cell loading. Activated monocytes incorporated CPNs in a higher proportion than monocytes in their naive state, without a loss of cellular functionality. In vitro PDT efficacy using cell-mediated delivery was superior to that using non vehiculized CPNs. Conclusion: CPN-loaded monocytes could efficiently deliver CPNs into GBM spheroids and the orthotopic model. Improved PDT in spheroids was confirmed using this delivery strategy.

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Citations

Dec 29, 2020·Journal of Neuroscience Research·Luis Exequiel IbarraLaura Natalia Milla Sanabria
Jan 4, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luís P FerreiraJoão F Mano
Oct 7, 2020·Journal of Photochemistry and Photobiology. B, Biology·Matías Daniel CaverzánLuis Exequiel Ibarra
May 16, 2021·Journal of Biomechanical Engineering·Zheng FangBing Zhang
Aug 6, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·D MendanhaN M Neves

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Methods Mentioned

BETA
PMA
reverse transcription PCR
fluorescence microscopy
flow cytometry
confocal microscopy
Fluorescence
fluorescence imaging

Software Mentioned

Coloc
Primer BLAST
Strata gene MxPro QPCR
ImageJ Plot Profile
Fiji

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