Trolox and ascorbic acid reduce direct and indirect oxidative stress in the IPEC-J2 cells, an in vitro model for the porcine gastrointestinal tract

PloS One
Hans VergauwenChris Van Ginneken

Abstract

Oxidative stress in the small intestinal epithelium is a major cause of barrier malfunction and failure to regenerate. This study presents a functional in vitro model using the porcine small intestinal epithelial cell line IPEC-J2 to examine the effects of oxidative stress and to estimate the antioxidant and regenerative potential of Trolox, ascorbic acid and glutathione monoethyl ester. Hydrogen peroxide and diethyl maleate affected the tight junction (zona occludens-1) distribution, significantly increased intracellular oxidative stress (CM-H2DCFDA) and decreased the monolayer integrity (transepithelial electrical resistance and FD-4 permeability), viability (neutral red) and wound healing capacity (scratch assay). Trolox (2 mM) and 1 mM ascorbic acid pre-treatment significantly reduced intracellular oxidative stress, increased wound healing capacity and reduced FD-4 permeability in oxidatively stressed IPEC-J2 cell monolayers. All antioxidant pre-treatments increased transepithelial electrical resistance and viability only in diethyl maleate-treated cells. Glutathione monoethyl ester (10 mM) pre-treatment significantly decreased intracellular oxidative stress and monolayer permeability only in diethyl maleate-treated cells. ...Continue Reading

References

Sep 1, 1987·Experimental Eye Research·A SpectorS Chifflet
Jul 1, 1967·The Biochemical Journal·E Boyland, L F Chasseaud
Dec 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D A SamarasingheG C Farrell
Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·R K RaoA Gupta
Feb 27, 2003·Biochemical and Biophysical Research Communications·G KaleR K Rao
Apr 14, 2006·Current Medicinal Chemistry·P ZafrillaJ M Morillas
Nov 2, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Bettina EbertAlfonso Lampen
Mar 21, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Aziz GuellichCatherine Coirault
Apr 4, 2007·Digestive Diseases and Sciences·Ali RezaieMohammad Abdollahi
Apr 13, 2007·Physiological Reviews·Anthony T BlikslagerJack Odle
Jun 29, 2007·Digestive Diseases and Sciences·Theresa A HerringJanos Zempleni
Jul 5, 2008·Nature Protocols·Guillermo RepettoJorge L Zurita
May 19, 2009·Veterinary Immunology and Immunopathology·Valentina MarianiElisabetta Giuffra
Aug 15, 2009·European Journal of Nutrition·Rashmi Santosh Tupe, Vaishali Vilas Agte
Dec 22, 2009·Inflammatory Bowel Diseases·Kimberley LewisDerek M McKay
Feb 8, 2011·Antioxidants & Redox Signaling·Lena J JohnJörg-Dieter Schulzke
Apr 27, 2011·Human Molecular Genetics·Mairead Kelly-AubertAleksander Edelman
May 4, 2011·Journal of Food Science·Hugo Palafox-CarlosGustavo A González-Aguilar
Jul 22, 2011·Journal of Visualized Experiments : JoVE·Maria Pia TestaTom R Phillips
Aug 27, 2011·Revista Española De Enfermedades Digestivas : Organo Oficial De La Sociedad Española De Patología Digestiva·Diego S FagundesMa Divina Murillo
Nov 15, 2011·Veterinary Microbiology·Amanda J Brosnahan, David R Brown
Mar 1, 2012·World Journal of Gastrointestinal Pathophysiology·Linda Chia-Hui YuYen-Hsuan Ni
Apr 10, 2012·Seminars in Cell & Developmental Biology·Magdalena L Circu, Tak Yee Aw
Jul 18, 2012·Bulletin of Experimental Biology and Medicine·E V OzhegovS S Timoshin
Jul 31, 2012·Journal of Cancer Research and Therapeutics·Anil KumarShashi Khandelwal

❮ Previous
Next ❯

Citations

Jul 26, 2016·Frontiers in Physiology·Sabine SchindeleMartin Diener
Nov 16, 2016·Antioxidants·Hans VergauwenChris van Ginneken
Nov 26, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yichun GuanWenjie Zhao
Aug 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhigang ChenJianming Su
Jun 22, 2019·Physiological Reports·Xudong WangKenneth McCormick
Dec 17, 2019·Acta Physiologica·Rizwan QaisarAdel B Elmoselhi
Oct 31, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei WangJoris Michiels
Mar 9, 2019·Frontiers in Veterinary Science·Xia XiongYanhong Liu
Jun 4, 2020·Animals : an Open Access Journal From MDPI·Tamil Selvi SundaramAntonella Baldi
Apr 26, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Čedomil Lucu
Oct 7, 2020·International Journal of Molecular Sciences·Miriam AyusoChris Van Ginneken
Mar 2, 2021·Frontiers in Veterinary Science·Lan ZhengSung Woo Kim
May 6, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mark H ManninoJonathan S Fisher
Mar 19, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Miriam AyusoChris Van Ginneken
Apr 29, 2021·Journal of Cellular Biochemistry·Tomokazu OhnishiTetsuya Matsuguchi

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry

Software Mentioned

analySIS
Pro
FlowJo
Magellan
SPSS
SPSS Statistics

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.