Abstract
The cell surface glycoprotein Trop2 is overexpressed in various types of epithelial cancer. Laryngeal carcinoma is one of the most common types of head and neck cancer and in a previous study, the expression of Trop2 in laryngeal squamous cell carcinoma (LSCC) was identified as an independent prognostic factor. However, the biological significance of Trop2 in LSCC development remains unclear. In the current study, Trop2 protein expression in fresh LSCC tissue and paracancerous tissue was investigated using western blotting. Trop2 in the Hep2 laryngeal cell line was subsequently suppressed by transfection with small interfering RNA (siRNA). The effects of knockdown of Trop2 on cell viability, migration, invasiveness and ERK/MAPK pathway activity were investigated in the current study. The expression of Trop2 in fresh LSCC tissue was demonstrated to be significantly greater than that in paracancerous tissue. Trop2 expression was also identified to be required for proliferation, migration and invasiveness of Hep2 laryngeal carcinoma cells, as all were blocked by siRNA-mediated Trop2 inhibition. Notably, the ERK/MAPK signaling pathway and cell cycle factor, cyclin D1, were identified to be suppressed following the knockdown of Trop...Continue Reading
References
Apr 7, 1999·Nature Genetics·M TsujikawaY Nakamura
Oct 12, 2000·The Biochemical Journal·W Kolch
Dec 12, 2000·The Journal of Biological Chemistry·L B GardnerC V Dang
Apr 18, 2001·Cytogenetics and Cell Genetics·G CalabreseS Alberti
Dec 18, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Dominic FongKlaus Laimer
Jun 24, 2008·Otolaryngologic Clinics of North America·Eugene A Chu, Young J Kim
Sep 25, 2008·British Journal of Cancer·D FongG Spizzo
Nov 21, 2008·Journal of Cellular Biochemistry·Kayoko NishiRobert H Rice
May 8, 2009·International Journal of Colorectal Disease·Y J FangD S Wan
Sep 15, 2010·Expert Review of Anticancer Therapy·Piet DirixSandra Nuyts
Sep 23, 2010·Molecular Cancer·Rafael CubasQizhi Yao
Jan 13, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Feb 22, 2012·Oncogene·M TrerotolaS Alberti
May 9, 2012·Oncogene·E GuerraS Alberti
Nov 15, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shailendra Kapoor
Feb 19, 2013·Oncogene·H NakanishiC M Croce
Oct 3, 2013·PloS One·Ting LiuXingsheng Yang
Citations
Oct 1, 2017·Experimental and Therapeutic Medicine·Bin WuXingsheng Yang
Oct 23, 2019·International Journal of Molecular Medicine·Genxiong TangZhengqing Feng
Aug 29, 2020·BMC Cancer·Baoping ZhangKailiang Zhang
Jul 14, 2018·Cancer Cell International·Stephanie M ZimmersKathleen F Arcaro
Jul 16, 2017·BMC Cancer·Hongyu GuanYanbing Li