Trop2 inhibition suppresses the proliferation and invasion of laryngeal carcinoma cells via the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway

Molecular Medicine Reports
Xudong WangHao Wu

Abstract

The cell surface glycoprotein Trop2 is overexpressed in various types of epithelial cancer. Laryngeal carcinoma is one of the most common types of head and neck cancer and in a previous study, the expression of Trop2 in laryngeal squamous cell carcinoma (LSCC) was identified as an independent prognostic factor. However, the biological significance of Trop2 in LSCC development remains unclear. In the current study, Trop2 protein expression in fresh LSCC tissue and paracancerous tissue was investigated using western blotting. Trop2 in the Hep2 laryngeal cell line was subsequently suppressed by transfection with small interfering RNA (siRNA). The effects of knockdown of Trop2 on cell viability, migration, invasiveness and ERK/MAPK pathway activity were investigated in the current study. The expression of Trop2 in fresh LSCC tissue was demonstrated to be significantly greater than that in paracancerous tissue. Trop2 expression was also identified to be required for proliferation, migration and invasiveness of Hep2 laryngeal carcinoma cells, as all were blocked by siRNA-mediated Trop2 inhibition. Notably, the ERK/MAPK signaling pathway and cell cycle factor, cyclin D1, were identified to be suppressed following the knockdown of Trop...Continue Reading

References

Dec 12, 2000·The Journal of Biological Chemistry·L B GardnerC V Dang
Dec 18, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Dominic FongKlaus Laimer
Jun 24, 2008·Otolaryngologic Clinics of North America·Eugene A Chu, Young J Kim
Sep 25, 2008·British Journal of Cancer·D FongG Spizzo
Sep 15, 2010·Expert Review of Anticancer Therapy·Piet DirixSandra Nuyts
Jan 13, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
May 9, 2012·Oncogene·E GuerraS Alberti
Nov 15, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shailendra Kapoor

❮ Previous
Next ❯

Citations

Oct 1, 2017·Experimental and Therapeutic Medicine·Bin WuXingsheng Yang
Oct 23, 2019·International Journal of Molecular Medicine·Genxiong TangZhengqing Feng
Jul 14, 2018·Cancer Cell International·Stephanie M ZimmersKathleen F Arcaro

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence
transfection

Software Mentioned

SPSS
Quantity One 1 - D Analysis

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

© 2021 Meta ULC. All rights reserved