Trophic effects of adipose-tissue-derived and bone-marrow-derived mesenchymal stem cells enhance cartilage generation by chondrocytes in co-culture

PloS One
M M PleumeekersG J V M van Osch

Abstract

Combining mesenchymal stem cells (MSCs) and chondrocytes has great potential for cell-based cartilage repair. However, there is much debate regarding the mechanisms behind this concept. We aimed to clarify the mechanisms that lead to chondrogenesis (chondrocyte driven MSC-differentiation versus MSC driven chondroinduction) and whether their effect was dependent on MSC-origin. Therefore, chondrogenesis of human adipose-tissue-derived MSCs (hAMSCs) and bone-marrow-derived MSCs (hBMSCs) combined with bovine articular chondrocytes (bACs) was compared. hAMSCs or hBMSCs were combined with bACs in alginate and cultured in vitro or implanted subcutaneously in mice. Cartilage formation was evaluated with biochemical, histological and biomechanical analyses. To further investigate the interactions between bACs and hMSCs, (1) co-culture, (2) pellet, (3) Transwell® and (4) conditioned media studies were conducted. The presence of hMSCs-either hAMSCs or hBMSCs-increased chondrogenesis in culture; deposition of GAG was most evidently enhanced in hBMSC/bACs. This effect was similar when hMSCs and bAC were combined in pellet culture, in alginate culture or when conditioned media of hMSCs were used on bAC. Species-specific gene-expression analy...Continue Reading

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Citations

Feb 18, 2020·Frontiers in Cell and Developmental Biology·Maria Jose Ramirez-BajoFritz Diekmann
Sep 6, 2020·Molecular Biology Reports·Anupama KakkarSiddharth Pandey
Nov 18, 2020·Regenerative Medicine·Jun ZhangYi Liu
Jan 30, 2021·Biofabrication·Marian FürsatzSylvia Nürnberger
Jul 20, 2021·Stem Cell Research & Therapy·Weitao ZhengMingjian Bei

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Methods Mentioned

BETA
biopsy
dissection
Polymerase Chain Reaction
biopsies

Software Mentioned

Basic Local Alignment Search Tool ( BLASTN )
SDS
PrimerExpress
Matlab® script

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