Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons

Neuroscience
B M GirardR L Parsons

Abstract

The present study investigated the influence of trophic factors on the expression of cocaine- and amphetamine-regulated transcript peptide (CARTp) in guinea-pig cardiac ganglia maintained in explant culture. In acutely isolated cardiac ganglia preparations, <1% of the cholinergic cardiac neurons exhibited CARTp immunoreactivity. In contrast, this number increased to >25% of the cardiac neurons after 72 h in explant culture. This increase in the number of CARTp neurons in cultured cardiac ganglia explants was accompanied by an increase in CARTp transcript levels as assessed by real time polymerase chain reaction. Treatment of cardiac ganglia cultures with neurturin or glial-derived trophic factor (both at 10 ng/ml) for 72 h prevented the increase in neurons that exhibited CARTp immunoreactivity. In contrast, treatment with ciliary neurotrophic factor (50 ng/ml) for 72 h produced a small significant increase in the percentage of CARTp-immunoreactive cardiac neurons and treatment with nerve growth factor (100 ng/ml) had no effect. Neurturin treatment also decreased cardiac neuron CARTp levels after 72 h in explant culture. Cardiac neurons exhibited immunoreactivity to the neurturin receptor GFRalpha2 whereas non-neural cells prefe...Continue Reading

References

Jan 1, 1994·Trends in Neurosciences·T HökfeltZ Wiesenfeld-Hallin
Aug 1, 1996·Cell and Tissue Research·G M MaweR L Parsons
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·D J CreedonE M Johnson
Jan 31, 1998·The Journal of Biological Chemistry·S JingG M Fox
Oct 6, 1998·Journal of the Autonomic Nervous System·A L KennedyR L Parsons
May 13, 1999·The Journal of Comparative Neurology·J LegerF M Smith
Jun 17, 1999·Trends in Neurosciences·M J Kuhar, S E Dall Vechia
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·C BrobergerT Hökfelt
Dec 29, 1999·Brain Research·L D AdamsM J Kuhar
Apr 20, 2000·Regulatory Peptides·M J KuharY Smith
Sep 7, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J O HiltunenM Saarma
Aug 25, 2001·Annual Review of Neuroscience·M V SofroniewW C Mobley
May 4, 2002·Nature Reviews. Neuroscience·Matti S Airaksinen, Mart Saarma
Jul 31, 2002·Neuropeptides·M J KuharB Balkan
Apr 16, 2004·Journal of Neurobiology·Kristen L BoeshoreRichard E Zigmond

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Citations

Apr 18, 2014·Journal of Molecular Neuroscience : MN·K ZvarovaM A Vizzard
Oct 5, 2007·American Journal of Physiology. Cell Physiology·Jennifer L HoardRobert Wondergem

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