Trophic factors and neuronal interactions regulate the cell cycle and Pax6 expression in Müller stem cells

Journal of Neuroscience Research
M Fernanda InsuaLuis E Politi

Abstract

The finding that Müller cells have stem cell properties in the retina has led to the hypothesis that they might be a source for replacing neurons lost in neurodegenerative diseases. However, utilization of Müller cells for regenerative purposes in the mammalian eye still requires identifying those factors that regulate their multipotentiality and proliferation. In addition, because Pax6 expression is indispensable for eye development, its regulation would be required during regeneration. In the present study we investigated the regulation of cell-cycle progression and Pax6 expression in pure Müller glial cell cultures and neuroglial cocultures from rat retinas. At early times in vitro, glial cells showed high expression of Pax6 and of nestin, a stem cell marker, and of markers of cell-cycle progression; expression of these markers decreased during development in parallel with increased glial differentiation. The addition of glial-derived neurotrophic factor, basic fibroblast growth factor, and insulin restored proliferation and also Pax6 and nestin expression in glial cells. Noteworthy, in neuroglial cocultures Müller cells retained Pax6 expression for longer periods, and, in turn, neuronal progenitors preserved their prolifera...Continue Reading

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Citations

Dec 1, 2010·The Journal of Veterinary Medical Science·Safwat Ali Mohamed AliMasato Uehara
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mike O KarlThomas A Reh
Oct 16, 2008·Journal of Neuroscience Research·Carolina E AbrahanNora P Rotstein
Dec 18, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Honghua YuDong Feng Chen
Jan 15, 2015·Experimental Eye Research·Qian JianZheng Qin Yin
Apr 29, 2020·Annual Review of Vision Science·Manuela LahnePeter F Hitchcock
Nov 5, 2020·Experimental Eye Research·Tamara SotoLuis E Politi

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