Trophoblast differentiation in vitro: establishment and characterisation of a serum-free culture model for murine secondary trophoblast giant cells

Reproduction : the Official Journal of the Society for the Study of Fertility
Ahmed H K El-Hashash, S J Kimber

Abstract

Differentiation of trophoblast giant cells is an early event during the process of murine embryo implantation. However, differentiation of secondary trophoblast giant cells in the rodent is still only partially understood, probably because of the lack of suitable in vitro models and cell markers. In order to advance our understanding of trophoblast differentiation, suitable in vitro models and markers are required to study their development. The objectives of this study were to establish and characterise a serum-free in vitro model for murine secondary trophoblast cells. Secondary trophoblast giant cells growing in vitro and paraffin sections of day 8.5 postcoitum mouse embryos were processed for immunostaining to establish the expression of potential markers using antibodies to blood group antigens, E-cadherin, alpha(7) integrins and activator protein-gamma, as well as placental lactogen-II. Within 3 days in serum-free culture, ectoplacental cone-derived secondary trophoblast cells underwent simultaneous induction of both morphological and functional differentiation. Secondary trophoblasts grew in vitro as a monolayer of cells with giant nuclei and expressed B and Le-b/Le-y blood group antigens, alpha(7) integrins and placenta...Continue Reading

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Citations

Feb 17, 2006·Biology of Reproduction·Amy D GulticeThomas L Brown
May 29, 2012·Reproductive Biology and Endocrinology : RB&E·Katsuo KoshiKazuyoshi Hashizume
Sep 3, 2011·American Journal of Reproductive Immunology : AJRI·Alexandre U BorbelyEstela Bevilacqua
Mar 11, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Adam J WatkinsTom P Fleming
Sep 17, 2009·Mechanisms of Development·Ahmed H K El-HashashSusan J Kimber
Sep 26, 2009·Developmental Biology·Meaghan J Jones, Louis Lefebvre

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