Trophoblast stem cells rescue placental defect in SOCS3-deficient mice

The Journal of Biological Chemistry
Yutaka TakahashiJ N Ihle

Abstract

Stem cells have important clinical and experimental potentials. Trophoblast stem (TS) cells possess the ability to differentiate into trophoblast subtypes in vitro and contribute to the trophoblast lineage in vivo. Suppressor of cytokine signaling 3 (SOCS3) is a negative regulator of cytokine signaling. Targeted disruption of SOCS3 revealed embryonic lethality on E12.5; it was caused by placental defect with enhanced leukemia inhibitory factor receptor signaling. A complementation of the wild-type (WT) placenta by using tetraploid rescue technique showed that the embryonic lethality in SOCS3-deficient embryo was due to the placental defect. Here we demonstrate that TS cells supplementation rescues placental defect in SOCS3-deficient embryos. In the rescued placenta, TS cells were integrated into the placental structure, and a substantial structural improvement was observed in the labyrinthine layer that was disrupted in the SOCS3-deficient placenta. Importantly, by supplying TS cells, living SOCS3-deficient embryos were detected at term. These results indicate a functional contribution of TS cells in the placenta and their potential application.

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Citations

Nov 15, 2007·Stem Cells and Development·Catherine A VandevoortGordon C Douglas
Jul 15, 2009·The Keio Journal of Medicine·Akihiko Yoshimura
Jan 25, 2011·Placenta·S J RenaudM J Soares
Apr 6, 2007·Journal of Reproductive Immunology·Kristy Boyle, Lorraine Robb
Mar 3, 2009·BMB Reports·B Binas
May 12, 2012·Growth Factors·Jeffrey J Babon, Nicos A Nicola
Jul 10, 2012·Transcription·Anwarul Ferdous, Joseph A Hill
Jan 15, 2014·JAK-STAT·Pankaj SumanSatish Kumar Gupta

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