PMID: 9168473May 1, 1997Paper

Tropomyosin-2 cDNA lacking the 3' untranslated region riboregulator induces growth inhibition of v-Ki-ras-transformed fibroblasts

Molecular Biology of the Cell
R A Janssen, James W Mier

Abstract

The levels of high molecular weight isoforms of tropomyosin (TM) are markedly reduced in ras-transformed cells. Previous studies have demonstrated that the forced expression of tropomyosin-1 (TM-1) induces reversion of the transformed phenotype of ras-transformed fibroblasts. The effects of the related isoform TM-2 on transformation are less clear. To assess the effects of forced expression of the TM-2 protein on ras-induced tumorigenicity, we introduced a TM-2 cDNA lacking the 3' untranslated region riboregulator into ras-transformed NIH 3T3 fibroblasts. TM-2 expression resulted in a flatter cell morphology and restoration of stress fibers. TM-2 expression also significantly reduced growth rates in low serum, soft agar, and nude mice. The reduced growth rates were associated with a prolongation of G0-G1. To identify the mechanism of TM-2-induced growth inhibition, we analyzed the effects of TM-2 reexpression of ERK and c-jun N-terminal kinase (JNK) activities. Levels of ERK phosphorylation and activity in TM-2-transfected tumor cells were comparable to those in mock-transfected tumor cells. JNK activity was only modestly increased in ras-transformed cells relative to untransformed NIH 3T3 cells and only slightly reduced as res...Continue Reading

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Citations

Feb 13, 2001·Current Opinion in Genetics & Development·G Pawlak, D M Helfman
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
Nov 29, 2011·Journal of Structural Biology·Cheolwon ChoiDavid M Helfman
Oct 19, 2007·Seminars in Cancer Biology·G M O'NeillP W Gunning
Apr 20, 2004·International Journal of Cancer. Journal International Du Cancer·Geraldine PawlakDavid M Helfman
Nov 21, 1998·The Journal of Biological Chemistry·R A JanssenJ W Mier
Mar 17, 2007·The Journal of Biological Chemistry·Shuomin ZhuYin-Yuan Mo

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