Troponin T isoforms alter the tolerance of transgenic mouse cardiac muscle to acidosis

Archives of Biochemistry and Biophysics
Thomas M NosekJian-Ping Jin

Abstract

Troponin T (TnT) is an essential protein in the Ca2+ regulatory system of striated of muscle. Three fiber type-specific TnT genes have evolved in higher vertebrates to encode cardiac, slow and fast skeletal muscle TnT isoforms. To understand the functional significance of TnT isoforms, we studied the effects of acidosis on the contractility of transgenic mouse cardiac muscle that expresses fast skeletal muscle TnT. Contractility analysis of intact cardiac muscle strips showed that while no differences were detected at physiological pH, the transgenic cardiac muscle had significantly greater decreases in +dF/dtmax at acidic pH than that of the wild-type control. Contractility of skinned cardiac muscles demonstrated that the presence of fast TnT resulted in significantly larger decreases in force and Ca2+ sensitivity at acidic pH than that of the wild-type control. The effect of TnT isoforms on the tolerance of muscle to acidosis may explain the higher tolerance of embryonic versus adult cardiac muscles. The results are consistent with the hypothesis that charge differences in TnT isoforms contribute to the contractility of muscle. The data further support a hypothesis that slow TnT is similar to the cardiac, but not fast, and Tn...Continue Reading

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Citations

Sep 15, 2012·The Journal of Biological Chemistry·Jose Renato PintoJames D Potter
Jun 20, 2007·BMC Developmental Biology·Marco A CampinhoDeborah M Power
Feb 5, 2014·Biochemistry. Biokhimii︠a︡·I A Katrukha
Oct 19, 2016·Medicine and Science in Sports and Exercise·Edward P DeboldThomas M Nosek
Feb 15, 2012·Medicine and Science in Sports and Exercise·Edward P Debold
Sep 29, 2012·Journal of Applied Physiology·Edward P DeboldMatthew A Turner
Oct 23, 2010·Archives of Biochemistry and Biophysics·Bin Wei, J-P Jin

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