Troubleshooting in animal models of colitis: The use of a novel electrocautery model

Journal of Pharmacological and Toxicological Methods
Fadi H MouradNayef E Saadé

Abstract

Experimental colitis induced by chemical agents leads to upregulation of inflammatory cytokines in distant unaffected small intestine and to a decrease in nutrient absorption. To preclude any possible proximal diffusion of these chemicals, we designed a novel method for ulcer induction in the colon by electrocautery. Under light anesthesia, a colonic ulcer was induced in rats by a special electrocautery probe introduced in the descending colon through the rectum allowing the injection of a controlled electrolytic current. A direct current (3-7 mA) was delivered through the electrodes for 30s and then for another 30s after reversing the polarity of the electrodes. Then, the probe was moved for a distance of +/-0.5 cm and the current injection was repeated. Rats were sacrificed at various time intervals after ulcer induction (3-96 h). Samples from colon and jejunum were taken for histological assessment and determination, by ELISA, of the levels of interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha). In other groups of animals, jejunal amino acid absorption was determined in vivo at 24 and 48 h post electrocautery. A colonic ulcer persisted for 72 h after cauterization. A significant upregulation of the level...Continue Reading

References

Jan 13, 2000·Current Opinion in Immunology·R S BlumbergW Strober
Nov 23, 2000·British Journal of Pharmacology·N E SaadéS A Kanaan
Feb 28, 2002·Annual Review of Immunology·Warren StroberRichard S Blumberg
Jul 11, 2002·Journal of Gastroenterology·Toshifumi HibiAtsushi Sakuraba
Aug 27, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Fadi H MouradCamille F Nassar

❮ Previous
Next ❯

Citations

Feb 27, 2010·Journal of Pharmacological and Toxicological Methods·Michael J Curtis
Nov 1, 2016·Journal of Crohn's & Colitis·Fadi H MouradNayef E Saade
Jul 29, 2018·Scientific Reports·Sehyung PakSeung-Jae Myung

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.