Troubleshooting methods: Using intravital microscopy in drug research.

Journal of Pharmacological and Toxicological Methods
Ellen L Hughes, Felicity N E Gavins

Abstract

The technique of observing cellular interactions in real time using intravital microscopy is now a recognised technique in many laboratories. This review highlights the potential uses and pitfalls of this form of microscopy in drug research. This review covers topics such as applications of this highly versatile technique, the choice of vascular bed available (with particular emphasis on the cremaster muscle, the mesentery and the brain), and the types of measurements that can be collected. Where possible, we have provided examples and technical advice to aid the use of this technique in research. This article is not intended to be a complete overview of the use of intravital microscopy in drug research, and readers are encouraged to refer to the references engaged within.

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Citations

Nov 28, 2013·The Journal of Antimicrobial Chemotherapy·Samuel OrdenAngeles Alvarez
Sep 18, 2012·Current Opinion in Pharmacology·Felicity N E Gavins
Oct 26, 2010·Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography·Ana Cristina CamarozanoEliete Bouskela
Oct 12, 2010·Advanced Drug Delivery Reviews·Panomwat AmornphimolthamRoberto Weigert
Feb 27, 2010·Journal of Pharmacological and Toxicological Methods·Michael J Curtis
Feb 11, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Katia ConceiçãoMônica Lopes-Ferreira
Jan 4, 2012·Annals of the New York Academy of Sciences·Felicity N E Gavins
Sep 18, 2012·Microvascular Research·Constantino Carlos Reyes-AldasoroGillian M Tozer
Apr 22, 2015·Biomaterials·Sabine SellnerMarkus Rehberg
Jan 19, 2018·The Journal of Infectious Diseases·Victor Collado-DiazÁngeles Álvarez
Apr 21, 2020·The Journal of Investigative Dermatology·Patricia García-MartínezIsabel Andújar

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