Trout myomaker contains 14 minisatellites and two sequence extensions but retains fusogenic function.

The Journal of Biological Chemistry
Aurélie LandemaineJean-Charles Gabillard

Abstract

The formation of new myofibers in vertebrates occurs by myoblast fusion and requires fusogenic activity of the muscle-specific membrane protein myomaker. Here, using in silico (BLAST) genome analyses, we show that the myomaker gene from trout includes 14 minisatellites, indicating that it has an unusual structure compared with those of other animal species. We found that the trout myomaker gene encodes a 434-amino acid (aa) protein, in accordance with its apparent molecular mass (∼40 kDa) observed by immunoblotting. The first half of the trout myomaker protein (1-220 aa) is similar to the 221-aa mouse myomaker protein, whereas the second half (222-234 aa) does not correspond to any known motifs and arises from two protein extensions. The first extension (∼70 aa) apparently appeared with the radiation of the bony fish clade Euteleostei, whereas the second extension (up to 236 aa) is restricted to the superorder Protacanthopterygii (containing salmonids and pike) and corresponds to the insertion of minisatellites having a length of 30 nucleotides. According to gene expression analyses, trout myomaker expression is consistently associated with the formation of new myofibers during embryonic development, postlarval growth, and musc...Continue Reading

References

Aug 1, 1997·Cell and Tissue Research·A RowlersonA Veggetti
Sep 22, 2001·Current Biology : CB·M J BarresiS H Devoto
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Oct 27, 2006·The Journal of Endocrinology·Jean-Charles GabillardNuria Montserrat
Feb 23, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P SteinbacherW Stoiber
May 29, 2007·Nature Genetics·Bhylahalli P SrinivasSudipto Roy
Jan 15, 2008·Nature Protocols·Christine Thisse, Bernard Thisse
Mar 18, 2008·The Journal of Experimental Biology·Emmanuelle DumontPierre-Yves Rescan
Jul 31, 2008·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Pierre-Yves Rescan
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Regina Lee SohnEmanuela Gussoni
Feb 27, 2010·Bioinformatics·Matthieu MuffatoHugues Roest Crollius
Sep 3, 2010·Annual Review of Genetics·Rita GemayelKevin J Verstrepen
Nov 19, 2010·Cell and Tissue Research·Jean Charles GabillardGilles Paboeuf
Dec 20, 2011·PLoS Biology·Gareth T Powell, Gavin J Wright
Sep 4, 2012·Protein Engineering, Design & Selection : PEDS·Hirohito IshikawaZene Matsuda
Jul 23, 2013·Nature·Douglas P MillayEric N Olson
Dec 5, 2013·Physiological Reports·Peter J DurcanClaire E Stewart
May 20, 2014·Journal of Visualized Experiments : JoVE·Jacob Michael FroehlichPeggy R Biga
Aug 1, 2014·Biochemical and Biophysical Research Communications·Aurélie LandemaineJean-Charles Gabillard
Aug 3, 2014·Genes & Development·Douglas P MillayEric N Olson
Feb 7, 2015·The Journal of Experimental Biology·Pierre-Yves RescanMaxence Fretaud
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Douglas P MillayEric N Olson
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Feb 6, 2017·Developmental Biology·Weibin Zhang, Sudipto Roy
Apr 8, 2017·Science·Pengpeng BiEric N Olson
Jun 2, 2017·Nature Communications·Malgorzata E QuinnDouglas P Millay
Jul 7, 2017·Nature Communications·Silvio Alessandro Di GioiaElizabeth C Engle
Sep 2, 2017·The Journal of Biological Chemistry·Dilani G GamageDouglas P Millay
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jun ShiEric N Olson
Mar 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Pengpeng BiEric N Olson

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jun ShiEric N Olson
Genes & Development
Douglas P MillayEric N Olson
Proceedings of the National Academy of Sciences of the United States of America
Douglas P MillayEric N Olson
Proceedings of the National Academy of Sciences of the United States of America
Pengpeng BiEric N Olson
© 2022 Meta ULC. All rights reserved