PMID: 18202551Jan 19, 2008

Troy binding to lymphotoxin-alpha activates NF kappa B mediated transcription

Cell Cycle
Tsuyoshi HashimotoChang-Yi Cui

Abstract

Troy is a TNFR superfamily member that is highly expressed in developing hair follicles. Its possible function and ligand in skin have, however, been unknown. Here we demonstrate that an immunomodulatory cytokine, lymphotoxin-alpha (LTalpha), is a functional ligand of Troy by 3 biochemical approaches: (1) Immunoprecipitation assays revealed that LTalpha, but not LTbeta or any obligate combination of LTalpha and LTbeta, binds to Troy. (2) Co-transfection of LTalpha with Troy sharply upregulated NFkappaB reporter transcription, whereas LTbeta or a combination of LTalpha and LTbeta did not. (3) Recombinant LTalpha protein upregulated NFkappaB activity through Troy in a dosedependent manner. We further found that LTalpha is expressed in dermal papillae of developing hair follicles, whereas Troy was expressed in adjacent matrix region. This suggested involvement of LTalpha-Troy signaling in mesenchyme-epithelium interactions during hair follicle development. However, in Troy mutant mice that we generated, hair subtype composition and morphology were altered slightly if at all. The present study thus suggested a subtle function of the newly identified LTalpha-Troy pathway in skin appendage development, however, it may have an additio...Continue Reading

Citations

Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vincent DuheronChristopher G Mueller
Jul 27, 2011·Stem Cells and Development·Xusheng WangYaojiong Wu
Aug 12, 2008·Human Molecular Genetics·Johanna PispaMarja L Mikkola
Dec 14, 2011·Cytokine & Growth Factor Reviews·Caroline RemouchampsEmmanuel Dejardin
Oct 17, 2008·The Journal of Investigative Dermatology·Chang-Yi CuiDavid Schlessinger
May 23, 2008·Cytokine & Growth Factor Reviews·Marja L Mikkola
Mar 14, 2014·International Journal of Cancer. Journal International Du Cancer·Simon SchönAndreas Herbst
Sep 10, 2015·Nature Reviews. Molecular Cell Biology·Annie Ng, Nick Barker
Apr 27, 2016·Disease Models & Mechanisms·Hongjie Li, Heinrich Jasper
Oct 20, 2018·Oncogene·Xiujie LiuCheng He
Jan 7, 2014·The Journal of Biological Chemistry·Christine Kowalczyk-QuintasPascal Schneider
Jan 13, 2015·World Journal of Gastroenterology : WJG·Yue ZhaoYong-Ning Zhou
May 24, 2013·Molecular Cancer Research : MCR·Joseph C LoftusNhan L Tran

Related Concepts

Tnfrsf19 protein, mouse
Lymphotoxin-alpha3
Mice, Inbred C57BL
Mice, Mutant Strains
Plasma Protein Binding Capacity
Transcription, Genetic
Ig-EBP-1
Tumor Necrosis Factor Receptor
Hair Follicle Structure

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