TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment Epithelium.

Investigative Ophthalmology & Visual Science
Peter Y ZhaoLawrence J Rizzolo

Abstract

Calcium regulates many functions of the RPE. Its concentration in the subretinal space and RPE cytoplasm is closely regulated. Transient receptor potential (TRP) channels are a superfamily of ion channels that are moderately calcium-selective. This study investigates the subcellular localization and potential functions of TRP channels in a first-passage culture model of human fetal RPE (hfRPE). The RPE isolated from 15- to 16-week gestation fetuses were maintained in serum-free media. Cultures were treated with barium chloride (BaCl2) in the absence and presence of TRP channel inhibitors and monitored by the transepithelial electrical resistance (TER). The expression of TRP channels was determined using quantitative RT-PCR, immunoblotting, and immunofluorescence confocal microscopy. Barium chloride substantially decreased TER and disrupted cell-cell contacts when added to the apical surface of RPE, but not when added to the basolateral surface. The effect could be partially blocked by the general TRP inhibitor, lanthanum chloride (LaCl3, ~75%), or an inhibitor of calpain (~25%). Family member-specific inhibitors, ML204 (TRPC4) and HC-067047 (TRPV4), had no effect on basal channel activity. Expression of TRPC4, TRPM1, TRPM3, TRP...Continue Reading

Citations

Aug 19, 2015·Frontiers in Cellular Neuroscience·Mauricio A RetamalCarlos González
Nov 22, 2016·Journal of Cellular Physiology·Jacqueline Martínez-RendónRefugio García-Villegas
Jan 27, 2017·American Journal of Physiology. Renal Physiology·Brian J SirokyBradley P Dixon
Mar 19, 2019·PloS One·Steven J KleeneNancy K Kleene
Jun 3, 2016·Physiological Reviews·John P M WhiteIstvan Nagy
Jan 24, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuefang ZhouAlan Shiels
Feb 2, 2021·Frontiers in Cell and Developmental Biology·Daniela IntartagliaIvan Conte
Apr 13, 2020·Progress in Retinal and Eye Research·Luminita ParaoanIan Grierson
Sep 27, 2020·Experimental Eye Research·Brianna D GuarinoCharles K Thodeti

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