TRPA1 channels: expression in non-neuronal murine lung tissues and dispensability for hyperoxia-induced alveolar epithelial hyperplasia

Pflügers Archiv : European journal of physiology
Martina KannlerAlexander Dietrich

Abstract

Transient receptor potential A1 (TRPA1) channels were originally characterized in neuronal tissues but also identified in lung epithelium by staining with fluorescently coupled TRPA1 antibodies. Its exact function in non-neuronal tissues, however, is elusive. TRPA1 is activated in vitro by hypoxia and hyperoxia and is therefore a promising TRP candidate for sensing hyperoxia in pulmonary epithelial cells and for inducing alveolar epithelial hyperplasia. Here, we isolated tracheal, bronchial, and alveolar epithelial cells and show low but detectable TRPA1 mRNA levels in all these cells as well as TRPA1 protein by Western blotting in alveolar type II (AT II) cells. We quantified changes in intracellular Ca2+ ([Ca2+]i) levels induced by application of hyperoxic solutions in primary tracheal epithelial, bronchial epithelial, and AT II cells isolated from wild-type (WT) and TRPA1-deficient (TRPA1-/-) mouse lungs. In all cell types, we detected hyperoxia-induced rises in [Ca2+]i levels, which were not significantly different in TRPA1-deficient cells compared to WT cells. We also tested TRPA1 function in a mouse model for hyperoxia-induced alveolar epithelial hyperplasia. A characteristic significant increase in thickening of alveolar...Continue Reading

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Citations

Nov 2, 2019·Physiological Reviews·Karel TalaveraBernd Nilius
Apr 3, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Brenda J MarshMatthew G Drake
Jun 13, 2020·International Journal of Molecular Sciences·Zsófia HajnaZsuzsanna Helyes
Sep 27, 2018·Pharmaceuticals·Thomas R H BüchAchim Aigner

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Methods Mentioned

BETA
electrophoresis
reverse-transcription PCRs
PCR

Software Mentioned

Progenesis SameSpots
CAST
nCounter Digital Analyzer

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