TRPC1-mediated Ca²⁺ entry is essential for the regulation of hypoxia and nutrient depletion-dependent autophagy

Cell Death & Disease
P SukumaranBrij B Singh

Abstract

Autophagy is a cellular catabolic process needed for the degradation and recycling of protein aggregates and damaged organelles. Although Ca(2+) is suggested to have an important role in cell survival, the ion channel(s) involved in autophagy have not been identified. Here we demonstrate that increase in intracellular Ca(2+) via transient receptor potential canonical channel-1 (TRPC1) regulates autophagy, thereby preventing cell death in two morphologically distinct cells lines. The addition of DMOG or DFO, a cell permeable hypoxia-mimetic agents, or serum starvation, induces autophagy in both epithelial and neuronal cells. The induction of autophagy increases Ca(2+) entry via the TRPC1 channel, which was inhibited by the addition of 2APB and SKF96365. Importantly, TRPC1-mediated Ca(2+) entry resulted in increased expression of autophagic markers that prevented cell death. Furthermore, hypoxia-mediated autophagy also increased TRPC1, but not STIM1 or Orai1, expression. Silencing of TRPC1 or inhibition of autophagy by 3-methyladenine, but not TRPC3, attenuated hypoxia-induced increase in intracellular Ca(2+) influx, decreased autophagy, and increased cell death. Furthermore, the primary salivary gland cells isolated from mice ex...Continue Reading

References

Jan 1, 1981·Reviews of Physiology, Biochemistry and Pharmacology·A B Borle
Mar 1, 1994·Cancer Metastasis Reviews·K Cole, E Kohn
Feb 1, 2000·The Journal of Biological Chemistry·X LiuI S Ambudkar
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B B SinghI S Ambudkar
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Sep 28, 2005·The Journal of Cell Biology·Sovan SarkarDavid C Rubinsztein
Feb 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Esther BuytaertPatrizia Agostinis
Jan 25, 2007·Molecular Cell·Maria Høyer-HansenMarja Jäättelä
Jan 27, 2007·Cell Death and Differentiation·A CriolloG Kroemer
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jen LiouTobias Meyer
Oct 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xibao LiuIndu S Ambudkar
Mar 22, 2008·The Journal of Biological Chemistry·Kenjiro SakakiRandal J Kaufman
Oct 14, 2008·Cellular and Molecular Life Sciences : CMLS·S H WangC M Shih
Oct 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joel Abramowitz, Lutz Birnbaumer
Feb 10, 2009·Methods in Enzymology·Geir BjørkøyTerje Johansen
Mar 28, 2009·Cell Death and Differentiation·J M VicencioG Kroemer
Nov 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Biswaranjan PaniIndu S Ambudkar
Feb 2, 2010·Cellular Signalling·Antje GrotemeierBjörn Stork
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Johanna KnöferlePaul Lingor
Mar 24, 2010·The Journal of Biological Chemistry·M Tariq Khan, Suresh K Joseph
May 1, 2010·Autophagy·Annalisa GastaldelloMichelangelo Campanella
Jul 28, 2010·The FEBS Journal·Natalia B Pivovarova, S Brian Andrews
Sep 10, 2010·Cold Spring Harbor Perspectives in Biology·Michael W Harr, Clark W Distelhorst
Oct 21, 2010·Physiological Reviews·Brinda RavikumarDavid C Rubinsztein
Dec 15, 2010·Neuron·Takayasu HigoKatsuhiko Mikoshiba
Dec 31, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei JiaYou-Wen He
Feb 4, 2011·Advances in Experimental Medicine and Biology·Christoffer LöfKid Törnquist
Feb 4, 2011·Advances in Experimental Medicine and Biology·Kwong Tai ChengIndu S Ambudkar
May 17, 2011·Cell Calcium·Jean-Paul DecuypereJan B Parys
Jul 16, 2011·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Yue Zhang, Stuart K Calderwood
Jul 23, 2011·Nature Reviews. Cancer·Natalia PrevarskayaYaroslav Shuba
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Biswaranjan PaniBrij B Singh
Jan 21, 2012·Biochemical Society Transactions·Jeremy T Smyth, James W Putney
Oct 18, 2012·Annual Review of Pathology·Lyndsay Murrow, Jayanta Debnath
Oct 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sheng ZhangBurton M Wice
Dec 12, 2012·Current Molecular Medicine·S S SmailiY-T Hsu

❮ Previous
Next ❯

Citations

Mar 21, 2016·Cell Calcium·Pramod SukumaranBrij B Singh
Nov 24, 2016·Journal of Cellular and Molecular Medicine·Bao-Dong TangRui-Ping Pang
Jun 21, 2015·Journal of Dental Research·M Morgan-BathkeK H Limesand
Jan 15, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sumit SahniDes R Richardson
Oct 6, 2017·Autophagy·Artem KondratskyiNatalia Prevarskaya
Oct 28, 2017·The Journal of Biological Chemistry·Danielle KroutKate J Claycombe-Larson
Jan 25, 2018·Oncogenesis·Varvara PetrovaIvano Amelio
Nov 9, 2019·Molecular Genetics & Genomic Medicine·Xiao-Dan LinXue-Yi Xue
Dec 5, 2019·International Journal of Molecular Sciences·Rodrigo Portes UreshinoGustavo José da Silva Pereira
Nov 19, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pramod SukumaranBrij B Singh
Dec 14, 2016·Molecular Neurobiology·Yuyang SunBrij B Singh
Feb 8, 2018·Physiological Reviews·Natalia PrevarskayaYaroslav Shuba
Jun 10, 2020·Cellular and Molecular Neurobiology·Yuyang SunBrij B Singh
May 22, 2019·FASEB BioAdvances·Pramod SukumaranBrij B Singh
Jan 12, 2021·Frontiers in Molecular Neuroscience·Karolina Serwach, Joanna Gruszczynska-Biegala
Mar 5, 2021·Autophagy·Ruoxi ZhangDaolin Tang
Feb 2, 2017·Cell Calcium·Nadine Hempel, Mohamed Trebak
Jul 19, 2015·Journal of Cell Science·Shanshan LiYizheng Wang
Jun 4, 2021·Frontiers in Pharmacology·Chang-Bo ZhengXiaoqiang Yao
Jun 29, 2021·Frontiers in Oncology·María Paz SaldíasOscar Cerda
Aug 28, 2021·Cells·Pramod SukumaranBrij B Singh

❮ Previous
Next ❯

Software Mentioned

Origin
MicroSoft Excel
OriginLab
Graphpad prism
imageJ
pClamp

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms