TRPC5 activation kinetics are modulated by the scaffolding protein ezrin/radixin/moesin-binding phosphoprotein-50 (EBP50)

Journal of Cellular Physiology
A G Obukhov, Martha C Nowycky

Abstract

TRPC1-7 proteins are members of a family of mammalian non-specific cation channels that mediate receptor-operated, phospholipase Cbeta/Cgamma dependent Ca(2+) influx in various cell types. TRPC4 and TRPC5 form a subfamily within TRPCs. Uniquely in the TRPC family, these channels possess a C-terminal "VTTRL" motif that binds to PDZ-domains of the scaffolding protein, EBP50 (NHERF1; Tang et al., J Biol Chem 275:37559-37564). The functional effects of EBP50 on TRPC4/5 activity have not been investigated. We have cloned rat TRPC5 (rTRPC5), functionally expressed it in HEK293 cell, and studied channel regulation with patch-clamp techniques. Both rTRPC5 and its VTTRL deletion mutant (r5dV) were localized to the plasma membrane. rTRPC5 did not display any significant basal activity in unstimulated HEK293 cells. In cells co-expressing rTRPC5 and H1 histamine receptor, rTRPC5 current evoked by GTPgammaS or histamine developed in two phases: a slowly developing, small inward current was followed by a rapidly developing, transient, large inward current. Each phase had a characteristic non-linear current-voltage (I-V) relationship. Deletion of the VTTRL motif had no detectable effect on the biophysical properties of the channel. Co-express...Continue Reading

References

Aug 4, 1998·The EMBO Journal·S PhilippV Flockerzi
Jul 27, 1999·The Journal of Physiology·J WarnatA Cavalié
May 19, 2000·The Journal of Biological Chemistry·S PhilippV Flockerzi
Jun 6, 2000·The Journal of Biological Chemistry·M SchaeferG Schultz
Apr 13, 2001·Neuron·C StrübingD E Clapham
Oct 12, 2001·Biochemical and Biophysical Research Communications·P G SuhJ H Kim
Feb 22, 2002·The Journal of Biological Chemistry·Alexander G Obukhov, Martha C Nowycky
Mar 15, 2002·Cell·Craig MontellVeit Flockerzi
Aug 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nati HernandoHeini Murer
Aug 24, 2002·The Journal of Biological Chemistry·Moulay Driss RochdiJean-Luc Parent
Sep 19, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian HarteneckGünter Schultz
Nov 29, 2002·The Journal of Biological Chemistry·Silke JungTim D Plant
Jan 18, 2003·Brain Research. Molecular Brain Research·Antonio RiccioMenelas N Pangalos
May 27, 2003·Cell Calcium·Christian Harteneck
May 27, 2003·Cell Calcium·Tim D Plant, Michael Schaefer
Jul 15, 2003·The Journal of Biological Chemistry·Carsten StrübingDavid E Clapham

❮ Previous
Next ❯

Citations

May 20, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Tim D Plant, Michael Schaefer
Jul 27, 2005·Pflügers Archiv : European journal of physiology·Kirill KiselyovShmuel Muallem
May 9, 2007·Pflügers Archiv : European journal of physiology·Indu S Ambudkar, Hwei Ling Ong
Oct 9, 2009·The Journal of Biological Chemistry·Jessica SabourinBruno Constantin
Jan 31, 2008·The Journal of Biological Chemistry·Ken-ichi OtsuguroAlexander V Zholos
Apr 29, 2009·The Journal of General Physiology·Nathaniel T BlairDavid E Clapham
Sep 24, 2010·Cold Spring Harbor Perspectives in Biology·Maarten GeesBernd Nilius
Jul 29, 2011·The Journal of Pharmacology and Experimental Therapeutics·Saikat ChakrabortyAlexander G Obukhov
Feb 8, 2006·Annual Review of Physiology·Baruch Minke, Moshe Parnas
Apr 10, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Eman Al-ShawafDavid J Beech
Aug 21, 2012·World Journal of Biological Chemistry·Francesco MocciaFranco Tanzi
Jan 13, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dhananjay P ThakurMichael X Zhu
Oct 4, 2008·The Journal of Physiology·Ana GomisFélix Viana
Mar 21, 2007·Cell Calcium·Sylvie Cayouette, Guylain Boulay
May 28, 2011·Journal of Cellular Physiology·Sanjay KumarAlexander G Obukhov
Aug 16, 2005·Cell Calcium·Stine Falsig PedersenBernd Nilius
Apr 8, 2015·PloS One·Bing ShenXiaoqiang Yao
Feb 6, 2008·Journal of Cellular Physiology·Alexander G Obukhov, Martha C Nowycky
Apr 22, 2005·American Journal of Physiology. Cell Physiology·Mei Hong ZhuKi Whan Kim
Jul 13, 2006·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Jose RussoIrma H Russo
Jan 5, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aurélie VandebrouckBruno Constantin
Jul 7, 2007·Physiological Reviews·Mark Donowitz, Xuhang Li
Jul 8, 2011·American Journal of Physiology. Cell Physiology·Tae Sik SungInsuk So
Jan 30, 2020·Journal of Cellular Physiology·Michael DattiloKeith Williams
Jan 26, 2007·American Journal of Physiology. Cell Physiology·Wenyi ZhangBarbara A Miller
Dec 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Ursula StorchThomas Gudermann
Sep 3, 2020·Cells·Xingjuan ChenAlexander G Obukhov
Nov 23, 2018·Cells·Michael Mederos Y SchnitzlerUrsula Storch
Nov 9, 2010·Communicative & Integrative Biology·Klaus Groschner
Feb 1, 2020·Pharmacology & Therapeutics·Hongbo WangMichael X Zhu
Mar 28, 2021·Science Advances·Laura BernalKatharina Zimmermann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.