TRPC6-dependent Ca2+ signaling mediates airway inflammation in response to oxidative stress via ERK pathway.

Cell Death & Disease
Qing-Zi ChenJian-Hua Li

Abstract

Ozone (O3) plays an extremely important role in airway inflammation by generating reactive oxygen species (ROS) including hydrogen peroxide, then promoting redox actions and causing oxidative stress. Evidences indicate that TRPC6 (canonical transient receptor potential channel 6) is a redox-regulated Ca2+ permeable nonselective cation channel, but its role in the setting of oxidative stress-related airway inflammation remains unknown. Here, we found that both TRPC6-/- mice and mice pretreated with SAR7334, a potent TRPC6 inhibitor, were protected from O3-induced airway inflammatory responses. In vitro, both knockdown of TRPC6 expression with shRNA and TRPC6 blockage markedly attenuated the release of cytokines IL-6 and IL-8 induced by O3 or H2O2 in 16HBE cells (human bronchial epithelial cell line). Treatment with O3 or H2O2 enhanced TRPC6 protein expression in vivo and vitro. We also observed that TRPC6-dependent increase of intracellular Ca2+ concentration ([Ca2+]i) was triggered by H2O2, which consisted of the release from intracellular calcium store and the influx of extracellular Ca2+ and could be further strengthened by 6-h O3 exposure in both 16HBE cells and HBEpiCs (primary human bronchial epithelial cells). Moreover, w...Continue Reading

References

Jul 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Randolph L CortelingIan P Hall
Aug 2, 2005·Molecular and Cellular Biology·Alexander DietrichLutz Birnbaumer
Nov 9, 2007·Annual Review of Physiology·C William Davis, Burton F Dickey
Oct 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joel Abramowitz, Lutz Birnbaumer
Jan 16, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·Hee Sun ParkYong Chul Lee
Mar 13, 2009·The New England Journal of Medicine·Michael JerrettMichael Thun
Jun 30, 2009·Antioxidants & Redox Signaling·Gillian GroegerThomas G Cotter
Oct 22, 2009·American Journal of Respiratory Cell and Molecular Biology·Tricia K Finney-HaywardLouise E Donnelly
May 27, 2010·The Journal of Biological Chemistry·Sarabeth GrahamRong Ma
Apr 29, 2011·American Journal of Physiology. Cell Physiology·Sarabeth GrahamRong Ma
Oct 28, 2011·American Journal of Physiology. Renal Physiology·Eun Young KimStuart E Dryer
Feb 18, 2012·Nature Communications·Norbert WeissmannAlexander Dietrich
Jun 7, 2012·Expert Opinion on Therapeutic Patents·Delia PretiRiccardo Patacchini
May 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Otto LindemannAlbrecht Schwab
Sep 17, 2013·Journal of Cellular Physiology·Marc AndersonStuart E Dryer
May 27, 2014·Environmental Science & Technology·Jessica G CharrierCort Anastasio
Aug 22, 2015·Redox Biology·Agnes GörlachOlga Krizanova
Oct 27, 2015·Molecular Pharmacology·Nicole UrbanMichael Schaefer
May 27, 2017·Environmental Science and Pollution Research International·Daniela NuvoloneFabio Voller
Jul 4, 2017·Frontiers in Immunology·Monika MalczykAkylbek Sydykov
Nov 17, 2017·American Journal of Physiology. Cell Physiology·Li-Fen ZhouJian-Hua Li
Aug 21, 2018·Frontiers in Physiology·Stuart E Dryer, Eun Young Kim

❮ Previous
Next ❯

Citations

Feb 20, 2021·Frontiers in Cell and Developmental Biology·Lu LiuShengmei Zhu
Mar 30, 2021·Frontiers in Pharmacology·Alessandro RimessiPaolo Pinton
Apr 13, 2021·Frontiers in Pharmacology·Verena HofschröerAlbrecht Schwab

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
fluorescence microscopy
bronchoalveolar
enzyme-linked immunosorbent assay
Protein Assay
confocal microscopy

Software Mentioned

ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.