TRPM7 channel is regulated by magnesium nucleotides via its kinase domain

The Journal of General Physiology
Philippe DemeuseAndrea Fleig

Abstract

TRPM7 is a Ca(2+)- and Mg(2+)-permeable cation channel that also contains a protein kinase domain. While there is general consensus that the channel is inhibited by free intracellular Mg(2+), the functional roles of intracellular levels of Mg.ATP and the kinase domain in regulating TRPM7 channel activity have been discussed controversially. To obtain insight into these issues, we have determined the effect of purine and pyrimidine magnesium nucleotides on TRPM7 currents and investigated the possible involvement of the channel's kinase domain in mediating them. We report here that physiological Mg.ATP concentrations can inhibit TRPM7 channels and strongly enhance the channel blocking efficacy of free Mg(2+). Mg.ADP, but not AMP, had similar, albeit smaller effects, indicating a double protection against possible Mg(2+) and Ca(2+) overflow during variations of cell energy levels. Furthermore, nearly all Mg-nucleotides were able to inhibit TRPM7 activity to varying degrees with the following rank in potency: ATP > TTP > CTP > or = GTP > or = UTP > ITP approximately free Mg(2+) alone. These nucleotides also enhanced TRPM7 inhibition by free Mg(2+), suggesting the presence of two interacting binding sites that jointly regulate TRPM7...Continue Reading

References

Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Pusch, E Neher
Nov 9, 1994·Molecular and Cellular Biochemistry·T W Traut
Dec 26, 2001·Chemical Reviews·J A Adams
Apr 10, 2002·Nature Cell Biology·Loren W RunnelsDavid E Clapham
Aug 1, 2002·The Journal of General Physiology·J Ashot KozakMichael D Cahalan
Jan 1, 2003·The Journal of General Physiology·Mahealani K Monteilh-ZollerAndrea Fleig
Jan 28, 2003·Biophysical Journal·J Ashot Kozak, Michael D Cahalan
Jul 31, 2003·Cell·Carsten SchmitzAndrew M Scharenberg
Dec 31, 2003·Cell·Michelle AartsMichael Tymianski
Apr 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ryuichi TakezawaAndrea Fleig
Aug 3, 2004·Journal of Pharmacological Sciences·Toyohisa HananoRyuji Inoue
Oct 16, 2004·The Journal of Biological Chemistry·Maxim V Dorovkov, Alexey G Ryazanov
Nov 9, 2004·Trends in Pharmacological Sciences·Andrea Fleig, Reinhold Penner
Mar 23, 2005·The Journal of Biological Chemistry·Masayuki MatsushitaAngus C Nairn
Apr 22, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Christian Harteneck
Jul 13, 2005·The Journal of General Physiology·Jianmin JiangLixia Yue
Jul 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Meredith C HermosuraRalph M Garruto

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Citations

May 10, 2011·Acta Pharmacologica Sinica·Christine You-jin Bae, Hong-shuo Sun
Jul 24, 2012·Acta Pharmacologica Sinica·Christine You Jin Bae, Hong-shuo Sun
Nov 4, 2010·Nature Communications·Lillia V RyazanovaAlexey G Ryazanov
Jan 6, 2010·The Journal of Biological Chemistry·Koichi InoueZhi-Gang Xiong
Jun 30, 2007·The Journal of Biological Chemistry·Mingjiang LiLixia Yue
Feb 28, 2008·Current Opinion in Gastroenterology·Gary A Quamme
Jun 21, 2007·Annual Review of Biochemistry·Kartik Venkatachalam, Craig Montell
Feb 21, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Tomohiro NumataYasunobu Okada
Aug 3, 2007·The Journal of Clinical Investigation·Shmuel Muallem, Orson W Moe
Aug 16, 2012·Journal of Biomedical Science·Regina MacianskieneVida Gendviliene
Dec 15, 2010·Circulation Journal : Official Journal of the Japanese Circulation Society·Alvaro YogiRhian M Touyz
Jun 23, 2010·PloS One·Hsiang-Chin ChenLoren W Runnels
Dec 17, 2009·Cellular and Molecular Life Sciences : CMLS·Jeroen MiddelbeekFrank N van Leeuwen
Oct 19, 2014·Nucleic Acids Research·Inna A NikonorovaAlexey G Ryazanov
Mar 19, 2014·Pflügers Archiv : European journal of physiology·T HofmannV Chubanov
Mar 9, 2013·Cellular and Molecular Life Sciences : CMLS·Haijie YuAndrea Fleig
Sep 23, 2006·The Journal of Physiology·Roger C Hardie
May 19, 2007·The Journal of Physiology·Bret F Bessac, Andrea Fleig
Dec 17, 2015·Pflügers Archiv : European journal of physiology·Sebastian SchäferVladimir Chubanov
Nov 22, 2007·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Bruno Sontia, Rhian M Touyz
Dec 21, 2006·Seminars in Cell & Developmental Biology·Pam MassulloSantiago Partida-Sánchez
Nov 25, 2006·Archives of Biochemistry and Biophysics·Kanigula MubagwaRegina Macianskiene
Jul 18, 2014·Scientific Reports·Taku KaitsukaMasayuki Matsushita
Sep 17, 2009·Immunological Reviews·Michael D Cahalan, K George Chandy
Feb 6, 2010·Journal of Cellular and Molecular Medicine·Yukihisa HamaguchiShinsuke Nakayama
Aug 21, 2012·Basic & Clinical Pharmacology & Toxicology·Byung J KimSeon J Kim
Aug 26, 2010·British Journal of Pharmacology·R MacianskieneK Mubagwa
Feb 21, 2009·The Biochemical Journal·Scott W Crawley, Graham P Côté
Jul 31, 2014·European Journal of Cell Biology·Daan VisserKees Jalink
Mar 22, 2014·The Biochemical Journal·Jenny van der WijstDario R Alessi
May 27, 2014·Cell·Grigory KrapivinskyDavid E Clapham
Aug 12, 2014·Pflügers Archiv : European journal of physiology·Alexandre BouronJohannes Oberwinkler
Oct 1, 2011·Biomolecular Concepts·Jie SongEric Beitz

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Methods Mentioned

BETA
phosphotransferase
phosphotranferase

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