TRPM7 Is Involved in Volume Regulation in Salivary Glands

Journal of Dental Research
J M KimK Park

Abstract

Under hypotonic conditions, the regulatory volume decrease (RVD) is essential to maintain physiological homeostasis and functions in diverse biological systems. Intracellular Ca(2+) has been reported as an important mediator of this response, but the underlying Ca(2+) mechanism responsible for RVD is still controversial. Here we investigate the role of Ca(2+) in the RVD response using live-cell imaging, microspectrofluorimetry, and a patch-clamp technique. A typical RVD was observed in submandibular gland acinar cells after swelling in a hypotonic solution, whereas intracellular Ca(2+) chelation completely inhibited the RVD response. The incidence and magnitude of the Ca(2+) transient were proportional to the degree of hypotonicity of the extracellular medium, and there was a close relationship between intracellular Ca(2+) concentration and the volumetric changes of the cells. Notably, this response was mediated by Ca(2+)-induced Ca(2+) release, which is triggered by Ca(2+) influx via stretch-activated TRPM7 channels. Furthermore, we detected the generation of Cl(-) currents in the swelling acinar cells upon hypotonic stress, and the current profile matched that of the Ca(2+)-activated Cl(-) currents. A specific inhibitor of Cl...Continue Reading

References

Sep 1, 1992·The American Journal of Physiology·P A NegulescuT E Machen
Jan 1, 1990·The Journal of General Physiology·S Grinstein, J D Smith
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
May 1, 1995·The Journal of Physiology·J ArreolaT Begenisich
May 1, 1993·The Journal of Membrane Biology·T Kotera, P D Brown
Mar 1, 1996·The American Journal of Physiology·K StrangeP S Jackson
Jan 15, 1996·The Journal of Physiology·J ArreolaT Begenisich
Mar 1, 1997·In Vitro Cellular & Developmental Biology. Animal·D O QuissellJ T Turner
Aug 6, 1998·The Journal of Membrane Biology·T SpeakeP D Brown
Mar 3, 1999·The Journal of Membrane Biology·V UrbachB J Harvey
Dec 23, 2000·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·H TinelR K Kinne
Oct 19, 2004·The Journal of Biological Chemistry·Maite ArnigesMiguel A Valverde
Feb 16, 2005·Annual Review of Physiology·James E MelvinTed Begenisich
Jul 1, 2005·Pflügers Archiv : European journal of physiology·David M Cohen
Jan 24, 2007·Physiological Reviews·Bernd NiliusJohn A Peters
Jan 2, 2009·Nature·Chaoliang WeiHeping Cheng
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Dec 24, 2011·Progress in Biophysics and Molecular Biology·Jean-Marc Dubois, Béatrice Rouzaire-Dubois
Jul 23, 2013·Nature·Tsai-Wen ChenDouglas S Kim

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Citations

May 19, 2018·Pharmaceuticals·Benedikt FelsAlbrecht Schwab
Mar 1, 2018·Journal of Dental Research·J WonS B Oh
Dec 5, 2018·Journal of Dental Research·W QiaoY X Su
Oct 18, 2018·Journal of Molecular Endocrinology·Aran SonDong Min Shin
Jun 20, 2019·Annals of the New York Academy of Sciences·Gentian MusaFelix B Engel
Oct 30, 2020·Bioscience of Microbiota, Food and Health·Yoshiko AiharaTakahiro Adachi

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Methods Mentioned

BETA
Transfection
biosensor

Software Mentioned

PClamp
Scion image

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